基于选择性二氨基马来腈的双通道发射探针用于 Al 的检测及其在活细胞成像中的应用。
A selective diaminomaleonitrile-based dual channel emissive probe for Al and its application in living cell imaging.
机构信息
College of Chemistry and Materials Science, Langfang Normal University, Langfang 065000, PR China.
College of Chemistry and Materials Science, Langfang Normal University, Langfang 065000, PR China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2019 Apr 5;212:349-355. doi: 10.1016/j.saa.2019.01.031. Epub 2019 Jan 16.
A selective diaminomaleonitrile-based fluorescent probe for Al(L) has been synthesized and characterized. The sensing behavior of L towards different metal ions was investigated by fluorescent spectra and competition experiment. The results showed that L exhibited a dual channel emission (λ 522 & 558 nm) with yellow fluorescence in the presence of Al over other metal ions in EtOH solution. The probe L was bound to Al in a 1:2 stoichiometric manner as supported by Job's plot analysis. The binding constant was 9.55 × 10 M with the detection limit of 10.3 nM. The turn-on response was attributed to the inhibition of PET and CN isomerization after binding with Al while the dual emissions were due to ESIPT and ICT processes. In addition, the application of L for imaging Al in living Hela cells was achieved.
一种基于选择性二氨基马来腈的荧光探针已被合成并进行了表征。通过荧光光谱和竞争实验研究了 L 对不同金属离子的传感行为。结果表明，L 在乙醇溶液中对 Al 具有双通道发射（λ 522 和 558nm），呈现黄色荧光，而对其他金属离子则没有。探针 L 与 Al 以 1:2 的化学计量比结合，这一结果得到了 Job 分析的支持。结合常数为 9.55×10 M，检测限为 10.3nM。开环响应归因于与 Al 结合后 PET 和 CN 异构化的抑制，而双发射则归因于 ESIPT 和 ICT 过程。此外，L 还被用于在活的 Hela 细胞中成像 Al。
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