一种基于单个量子点的用于DNA甲基转移酶信号开启检测的纳米传感器。
A single quantum dot-based nanosensor for the signal-on detection of DNA methyltransferase.
作者信息
Ma Fei, Liu Wen-Jing, Tang Bo, Zhang Chun-Yang
机构信息
College of Chemistry, Chemical Engineering, Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular, Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan 250014, China.
出版信息
Chem Commun (Camb). 2017 Jun 22;53(51):6868-6871. doi: 10.1039/c7cc03736h.
We develop a single quantum dot (QD)-based nanosensor for the signal-on detection of DNA methyltransferase (MTase). By integration of single-molecule counting with the QD-based fluorescence resonance energy transfer (FRET), the proposed nanosensor can sensitively detect DNA MTase with a detection limit of as low as 0.002 U mL, and it can be further applied for inhibitor screening and accurate detection of DNA MTase in complex biological samples.
我们开发了一种基于单量子点（QD）的纳米传感器，用于对DNA甲基转移酶（MTase）进行信号开启检测。通过将单分子计数与基于量子点的荧光共振能量转移（FRET）相结合，所提出的纳米传感器能够以低至0.002 U/mL的检测限灵敏地检测DNA MTase，并且它还可进一步应用于抑制剂筛选以及复杂生物样品中DNA MTase的准确检测。
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