EnviroCORE, Department of Science and Health, Institute of Technology Carlow, Kilkenny Road, R93 V960 Co. Carlow, Ireland.
TE Laboratories Ltd. (TelLab), Loughmartin Business Park, Tullow, R93 N529 Co. Carlow, Ireland.
Molecules. 2019 Jan 18;24(2):339. doi: 10.3390/molecules24020339.
Arsenic contamination of drinking water is a global concern. Standard laboratory methods that are commonly used for arsenic detection in water, such as atomic absorption spectroscopy and mass spectroscopy, are not suitable for mass monitoring purposes. Autonomous microfluidic detection systems combined with a suitable colorimetric reagent could provide an alternative to standard methods. Moreover, microfluidic detection systems would enable rapid and cost efficient in situ monitoring of water sources without the requirement of laborious sampling. The aim of this study is to optimize a colorimetric method based on leucomalachite green dye for integration into a microfluidic detection system. The colorimetric method is based on the reaction of arsenic (III) with potassium iodate in acid medium to liberate iodine, which oxidizes leucomalachite green to malachite green. A rapid colour development was observed after the addition of the dye. Beer's law was obeyed in the range between 0.07⁻3 µg mL. The detection limit and quantitation limit were found to be 0.19 and 0.64 µg mL, respectively.
饮用水砷污染是一个全球性的问题。常用于水中砷检测的标准实验室方法，如原子吸收光谱法和质谱法，不适合大规模监测目的。结合适当的比色试剂的自主微流控检测系统可以为标准方法提供替代方案。此外，微流控检测系统将能够在无需繁琐采样的情况下，快速且经济高效地对水源进行原位监测。本研究的目的是优化基于隐色孔雀绿染料的比色法，将其集成到微流控检测系统中。比色法基于砷（III）在酸性介质中与碘酸钾反应释放碘，碘将隐色孔雀绿氧化为孔雀绿。加入染料后，颜色迅速发展。在 0.07⁻3 µg mL 范围内遵守比尔定律。检测限和定量限分别为 0.19 和 0.64 µg mL。
