Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan.
CSIRO Agriculture and Food, Canberra ACT 2601, Australia.
Int J Mol Sci. 2019 Jan 21;20(2):451. doi: 10.3390/ijms20020451.
Dormancy is the mechanism that allows seeds to become temporally quiescent in order to select the right time and place to germinate. Like in other species, in barley, grain dormancy is gradually reduced during after-ripening. Phosphosignaling networks in barley grains were investigated by a large-scale analysis of phosphoproteins to examine potential changes in response pathways to after-ripening. We used freshly harvested (FH) and after-ripened (AR) barley grains which showed different dormancy levels. The LC-MS/MS analysis identified 2346 phosphopeptides in barley embryos, with 269 and 97 of them being up- or downregulated during imbibition, respectively. A number of phosphopeptides were differentially regulated between FH and AR samples, suggesting that phosphoproteomic profiles were quite different between FH and AR grains. Motif analysis suggested multiple protein kinases including SnRK2 and MAPK could be involved in such a difference between FH and AR samples. Taken together, our results revealed phosphosignaling pathways in barley grains during the water imbibition process.
休眠是一种机制，它使种子暂时静止，以便选择合适的时间和地点发芽。与其他物种一样，大麦中的谷物休眠在后熟过程中逐渐减弱。通过对磷酸化蛋白的大规模分析，研究了大麦种子中的磷酸信号网络，以检查对后熟反应途径的潜在变化。我们使用了不同休眠水平的新鲜收获（FH）和后熟（AR）大麦种子。LC-MS/MS 分析在大麦胚中鉴定出 2346 个磷酸肽，其中 269 个和 97 个分别在吸胀过程中上调或下调。FH 和 AR 样品之间有许多磷酸肽存在差异调节，这表明 FH 和 AR 谷物之间的磷酸化蛋白质组图谱差异很大。基序分析表明，包括 SnRK2 和 MAPK 在内的多种蛋白激酶可能参与了 FH 和 AR 样品之间的这种差异。总之，我们的研究结果揭示了大麦种子在水分吸胀过程中的磷酸化信号通路。
