Caplan Y H, Levine B
University of Maryland, Department of Pathology, Baltimore 21201.
J Anal Toxicol. 1988 Sep-Oct;12(5):265-7. doi: 10.1093/jat/12.5.265.
The TDx fluorescence polarization immunoassays (FPIA) for some therapeutically monitored drugs (lidocaine, phenytoin, phenobarbital) were applied to the analysis of postmortem blood specimens. After an initial calibration using the appropriate TDx calibrators, blood bank blood fortified with known amounts of drug was analyzed by FPIA. Postmortem blood specimens containing the drugs, postmortem specimens not containing the drugs of interest (negatives), and randomly selected postmortem blood specimens fortified with known amounts of the drugs were analyzed by the FPIA and reference gas chromatographic (GC) methods. Concentrations determined in blood bank and postmortem blood specimens showed excellent correlation between the two methods and produced lines with slopes ranging from 0.98 to 1.08. In each case the correlation coefficient was greater than 0.97. Analysis of all negative specimens produced FPIA values below the limit of quantitation of the reference GC assay.
将用于某些治疗药物监测（利多卡因、苯妥英、苯巴比妥）的TDx荧光偏振免疫分析（FPIA）应用于死后血液标本的分析。使用适当的TDx校准品进行初始校准后，通过FPIA分析添加了已知量药物的血库血液。对含有这些药物的死后血液标本、不含有相关药物的死后标本（阴性样本）以及随机选择的添加了已知量药物的死后血液标本，采用FPIA和参考气相色谱（GC）方法进行分析。血库和死后血液标本中测定的浓度在两种方法之间显示出极好的相关性，所得直线斜率范围为0.98至1.08。在每种情况下，相关系数均大于0.97。所有阴性标本的分析结果显示，FPIA值低于参考GC测定的定量限。
