Characteristics of extracellular polymeric substances and soluble microbial products of activated sludge in a pulse aerated reactor.

This study aimed to investigate the stratification characteristics of extracellular polymeric substances (EPS) and the properties of soluble microbial products (SMP) of the activated sludge with pulse aeration. The activated sludge was acclimated with aeration on/off time of 5 min/10 min for 60 days. The results showed that both polysaccharides (PS) and proteins (PN) increased in the loosely bound EPS (LB-EPS) and the tightly bound EPS (TB-EPS) with the increase of operational time. Both the PN/PS ratio and the total LB-EPS increased in the later period of the pulse aerated acclimation process. There was an obvious positive correlation between sludge volume index (SVI) and LB-EPS (  = 0.871), mainly due to the PS in LB-EPS which was also significantly correlated with SVI (  = 0.954). A downward trend of SMP concentrations occurred at the end of acclimation which was opposite to the upward change of EPS contents. Two obvious fluorescence peaks were detected respectively in EPS and SMP by 3D-EEM fluorescence spectroscopy. Peak A was detected in both LB-EPS and TB-EPS, which was associated with tryptophan protein-like substances. Peak B representing humus carbon and carboxylic acids was mainly detected in SMP. The release of humus-like components in SMP from activated sludge was mainly in accordance with the dissolution and hydrolysis of PN in TB-EPS.