CRISPR/Cas9-mediated knockout of causes systemic lymphopenia with hypoplastic lymphoid organs in FVB mice.

Recombination activating gene-2 () plays a crucial role in the development of lymphocytes by mediating recombination of T cell receptors and immunoglobulins, and loss of causes severe combined immunodeficiency (SCID) in humans. knockout mice created using homologous recombination in ES cells have served as a valuable immunodeficient platform, but concerns have persisted on the specificity of -related phenotypes in these animals due to the limitations associated with the genome engineering method used. To precisely investigate the function of , we recently established a new knockout FVB mouse line ( ) manifesting lymphopenia by employing a CRISPR/Cas9 system at Center for Mouse Models of Human Disease. In this study, we further characterized their phenotypes focusing on histopathological analysis of lymphoid organs. mice showed no abnormality in development compared to their WT littermates for 26 weeks. At necropsy, gross examination revealed significantly smaller spleens and thymuses in mice, while histopathological investigation revealed hypoplastic white pulps with intact red pulps in the spleen, severe atrophy of the thymic cortex and disappearance of follicles in lymph nodes. However, no perceivable change was observed in the bone marrow. Moreover, our analyses showed a specific reduction of lymphocytes with a complete loss of mature T cells and B cells in the lymphoid organs, while natural killer cells and splenic megakaryocytes were increased in mice. These findings indicate that our mice show systemic lymphopenia with the relevant histopathological changes in the lymphoid organs, suggesting them as an improved -related immunodeficient model.