Nooranizadeh M H, Mogheiseh A, Kafi M, Sepehrimanesh M, Vaseghi H
Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Gastrointestinal and Liver Diseases Research Center, Gilan University of Medical Sciences, Rahst, Iran.
Lab Anim Res. 2018 Dec;34(4):211-215. doi: 10.5625/lar.2018.34.4.211. Epub 2018 Dec 31.
The following experiments were designed to examine the effect of serum of spayed dogs on superovulation response in mice and rats. In Experiment 1, female mice at diestrus (n=30) were divided into three equal groups and superovulated with either administration of 5 IU pregnant mare serum gonadotropin (PMSG) or recombinant follicle stimulating hormone (rFSH) (reducing dose from 2.5 to 0.5 IU) and 5 IU human chorionic gonadotropin (hCG) administered 48h later. Serum of spayed dogs was administered intraperitoneally at a reduced dose from 0.1 to 0.025 mL in a 48 h period. In Experiment 2, female rats (n=30) at diestrus stage were divided into three equal groups. Superovulation was induced using either 30 IU PMSG, or a dose reduced from 5 to 1 IU rFSH and 25 IU hCG administered 48h later. Serum of spayed dogs was administered in a reduced dose from 0.6 to 0.1 mL in a 48 hour period. Female mice and rats were mated 24 h following hCG administration. On day 14 after mating, animals were euthanized and ovarian sections were fixed for histopathological evaluation and corpus luteum (CL) counting. No significant difference observed in mean (±SEM) number of CLs between the PMSG group and the mice that received serum of spayed dog (10.4±1.3 vs 9.2±1.0). Mean (±SEM) number of CLs tended to be lower in rats that received serum of spayed dog than those of rats which received either PMSG or rFSH (15.1±1.9 vs 23.6±3.1 and 23.1±2.9, =0.06, respectively). In conclusion, serum of spayed dogs is able to induce a superovulatory response in mice and rats.
以下实验旨在研究去势犬血清对小鼠和大鼠超排卵反应的影响。在实验1中，处于动情间期的雌性小鼠（n = 30）被分为三组，每组数量相等，分别通过注射5 IU孕马血清促性腺激素（PMSG）或重组促卵泡激素（rFSH）（剂量从2.5 IU降至0.5 IU）进行超排卵处理，并在48小时后注射5 IU人绒毛膜促性腺激素（hCG）。在48小时内，以递减剂量（从0.1 mL降至0.025 mL）腹腔注射去势犬血清。在实验2中，处于动情间期的雌性大鼠（n = 30）被分为三组，每组数量相等。使用30 IU PMSG或剂量从5 IU降至1 IU的rFSH进行超排卵诱导，并在48小时后注射25 IU hCG。在48小时内，以递减剂量（从0.6 mL降至0.1 mL）注射去势犬血清。在注射hCG后24小时，将雌性小鼠和大鼠进行交配。交配后第14天，对动物实施安乐死，并将卵巢切片固定以进行组织病理学评估和黄体（CL）计数。PMSG组与接受去势犬血清的小鼠之间，CL的平均数量（±SEM）未观察到显著差异（10.4±1.3对9.2±1.0）。接受去势犬血清的大鼠的CL平均数量（±SEM）往往低于接受PMSG或rFSH的大鼠（15.1±1.9对23.6±3.1和23.1±2.9，P值分别为0.06）。总之，去势犬血清能够诱导小鼠和大鼠产生超排卵反应。
