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通过庚二酸二甲酯（DMP）交联将抗体共价固定在蛋白G磁珠上，关于抗原识别阻碍的数据。

Data on antigen recognition hindrance by antibodies covalently immobilized to Protein G magnetic beads by dimethyl pimelimidate (DMP) cross-linking.

作者信息

Caminha Marcelle A, de Lorena Virginia Maria B, de Oliveira Júnior Wilson, Perales Jonas, Carvalho Paulo C, Lima Diogo B, Cavalcanti Maria da Glória A M, Martins Sílvia M, Valente Richard H, Menna-Barreto Rubem F S

机构信息

Laboratório de Biologia Celular, IOC, Fiocruz, Rio de Janeiro, RJ, Brazil.

Laboratório de Toxinologia, IOC, Fiocruz, Rio de Janeiro, RJ, Brazil.

出版信息

Data Brief. 2018 Dec 21;22:516-521. doi: 10.1016/j.dib.2018.12.057. eCollection 2019 Feb.

DOI:10.1016/j.dib.2018.12.057
PMID:30671503
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6327068/
Abstract

The data presented herein is related to the article entitled " immunoproteome: calpain-like CAP5.5 differentially detected throughout distinct stages of human Chagas disease cardiomyopathy" [1]. Electrophoretic analyses under denaturing and reducing conditions indicate that covalent immobilization of human IgG to Protein G magnetic beads by cross-linking with 50 mM dimethyl pimelimidate hinders the recognition of antigens in immunoprecipitation assays.

摘要

本文所呈现的数据与题为《免疫蛋白质组：钙蛋白酶样CAP5.5在人类恰加斯病性心肌病不同阶段的差异检测》的文章[1]相关。变性和还原条件下的电泳分析表明，通过与50 mM二甲基哌啶二酸交联将人IgG共价固定到蛋白G磁珠上会阻碍免疫沉淀试验中对抗原的识别。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a4d/6327068/4844d7146266/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a4d/6327068/db8ac768053f/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a4d/6327068/4844d7146266/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a4d/6327068/db8ac768053f/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a4d/6327068/4844d7146266/gr2.jpg

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1
Data on antigen recognition hindrance by antibodies covalently immobilized to Protein G magnetic beads by dimethyl pimelimidate (DMP) cross-linking.通过庚二酸二甲酯（DMP）交联将抗体共价固定在蛋白G磁珠上，关于抗原识别阻碍的数据。
Data Brief. 2018 Dec 21;22:516-521. doi: 10.1016/j.dib.2018.12.057. eCollection 2019 Feb.
2
Trypanosoma cruzi immunoproteome: Calpain-like CAP5.5 differentially detected throughout distinct stages of human Chagas disease cardiomyopathy.克氏锥虫免疫蛋白组学：钙蛋白酶样 CAP5.5 在不同阶段的人类恰加斯病心肌病中差异检测。
J Proteomics. 2019 Mar 1;194:179-190. doi: 10.1016/j.jprot.2018.11.019. Epub 2018 Nov 29.
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Cross-Linking Antibodies to Beads Using Dimethyl Pimelimidate (DMP).使用二甲基庚二酸亚胺（DMP）将抗体与珠子交联。
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Autoimmunity in Chagas disease cardiopathy: biological relevance of a cardiac myosin-specific epitope crossreactive to an immunodominant Trypanosoma cruzi antigen.恰加斯病性心肌病中的自身免疫：与克氏锥虫免疫显性抗原发生交叉反应的心肌肌凝蛋白特异性表位的生物学相关性
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Troponin T autoantibodies correlate with chronic cardiomyopathy in human Chagas disease.心肌肌钙蛋白 T 自身抗体与人类恰加斯病的慢性心肌病相关。
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Changes in isotype composition and antigen recognition of anti-Trypanosoma cruzi antibodies from acute to chronic Chagas disease.从急性恰加斯病到慢性恰加斯病，抗克鲁斯锥虫抗体的同种型组成及抗原识别的变化。
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本文引用的文献

1
Trypanosoma cruzi immunoproteome: Calpain-like CAP5.5 differentially detected throughout distinct stages of human Chagas disease cardiomyopathy.克氏锥虫免疫蛋白组学：钙蛋白酶样 CAP5.5 在不同阶段的人类恰加斯病心肌病中差异检测。
J Proteomics. 2019 Mar 1;194:179-190. doi: 10.1016/j.jprot.2018.11.019. Epub 2018 Nov 29.
2
Reevaluating the Trypanosoma cruzi proteomic map: The shotgun description of bloodstream trypomastigotes.重新评估克氏锥虫蛋白质组图谱：血流型锥鞭毛体的鸟枪法描述。
J Proteomics. 2015 Feb 6;115:58-65. doi: 10.1016/j.jprot.2014.12.003. Epub 2014 Dec 20.
3
[I Latin American guidelines for the diagnosis and treatment of Chagas cardiomyopathy].
[《拉丁美洲恰加斯心肌病诊断与治疗指南》]
Arq Bras Cardiol. 2011;97(2 Suppl 3):1-48.
4
Silver-staining of proteins in polyacrylamide gels: a general overview.聚丙烯酰胺凝胶中蛋白质的银染：概述
Cell Mol Biol (Noisy-le-grand). 1994 Feb;40(1):57-75.
5
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.