Department of Kinesiology, Faculty of Applied Health Sciences, Brock University, 1812 Sir Isaac Brock Way, St. Catharines, ON, L2S 3A1, Canada.
Department of Health Science, Brock University, 1812 Sir Isaac Brock Way, St. Catharines, ON, Canada.
Calcif Tissue Int. 2019 Jun;104(6):582-590. doi: 10.1007/s00223-019-00524-1. Epub 2019 Jan 23.
This study compared sclerostin's response to impact versus no-impact high-intensity interval exercise in young men and examined the association between exercise-induced changes in sclerostin and markers of bone turnover and oxidative stress. Twenty healthy men (22.3 ± 2.3 years) performed two high-intensity interval exercise trials (crossover design); running on treadmill and cycling on cycle ergometer. Trials consisted of eight 1 min running or cycling intervals at ≥ 90% of maximal heart rate, separated by 1 min passive recovery intervals. Blood samples were collected at rest (pre-exercise), and 5 min, 1 h, 24 h, and 48 h following each trial. Serum levels of sclerostin, cross-linked telopeptide of type I collagen (CTXI), procollagen type I amino-terminal propeptide (PINP), thiobarbituric acid reactive substances (TBARS), and protein carbonyls (PC) were measured. There was no significant time or exercise mode effect for PINP and PC. A significant time effect was found for sclerostin, CTXI, and TBARS with no significant exercise mode effect and no significant time-by-mode interaction. Sclerostin increased from pre- to 5 min post-exercise (47%, p < 0.05) and returned to baseline within 1 h following the exercise. CTXI increased from pre- to 5 min post-exercise (28%, p < 0.05), then gradually returned to baseline by 48 h. TBARS did not increase significantly from pre- to 5 min post-exercise but significantly decreased from 5 min to 48 h post-exercise. There were no significant correlations between exercise-induced changes in sclerostin and any other marker. In young men, sclerostin's response to high-intensity interval exercise is independent of impact and is not related to changes in bone turnover and oxidative stress markers.
这项研究比较了骨硬化蛋白对冲击和非冲击高强度间歇运动的反应，并探讨了运动诱导的骨硬化蛋白变化与骨转换和氧化应激标志物之间的关系。20 名健康男性（22.3±2.3 岁）进行了两次高强度间歇运动试验（交叉设计）；在跑步机上跑步和在自行车测力计上骑自行车。试验由 8 个 1 分钟的跑步或自行车间隔组成，间隔≥90%的最大心率，间隔 1 分钟的被动恢复期。在休息时（运动前）以及每次试验后 5 分钟、1 小时、24 小时和 48 小时采集血液样本。测量血清骨硬化蛋白、I 型胶原交联端肽（CTX-I）、I 型前胶原氨基端前肽（PINP）、硫代巴比妥酸反应物质（TBARS）和蛋白羰基（PC）水平。PINP 和 PC 没有明显的时间或运动方式效应。骨硬化蛋白、CTX-I 和 TBARS 有明显的时间效应，没有明显的运动方式效应，也没有明显的时间-方式相互作用。骨硬化蛋白从运动前到运动后 5 分钟增加（47%，p<0.05），并在运动后 1 小时内恢复到基线。CTX-I 从运动前到运动后 5 分钟增加（28%，p<0.05），然后在 48 小时内逐渐恢复到基线。TBARS 从运动前到运动后 5 分钟没有显著增加，但从 5 分钟到 48 小时显著下降。骨硬化蛋白的变化与任何其他标志物之间没有显著相关性。在年轻男性中，骨硬化蛋白对高强度间歇运动的反应与冲击无关，与骨转换和氧化应激标志物的变化无关。
