Department of Physical Education & Sport Science, University of Thessaly, Karies, Greece.
Institute of Human Performance and Rehabilitation, Centre for Research and Technology Thessaly, Greece.
J Sports Sci Med. 2018 Aug 14;17(3):501-508. eCollection 2018 Sep.
A single bout of exercise can result in inflammatory responses, increased oxidative stress and upregulation of enzymatic antioxidant mechanisms. Although low-volume high-intensity interval training (HIIT) has become popular, its acute responses on the above mechanisms have not been adequately studied. The present study evaluated the effects of HIIT on hematological profile and redox status compared with those following traditional continuous aerobic exercise (CET). Twelve healthy young men participated in a randomized crossover design under HIIT and CET. In HIIT session, participants performed four 30-sec sprints on a cycle-ergometer with 4 min of recovery against a resistance of 0.375 kg/kg of body mass. CET consisted of 30-min cycling on a cycle-ergometer at 70% of their VO. Blood was drawn at baseline, immediately post, 24h, 48h and 72h post-exercise and was analyzed for complete blood count and redox status (thiobarbituric acid reactive substances, [TBARS]; protein carbonyls, [PC]; total antioxidant capacity, [TAC]; catalase and uric acid). White blood cells (WBC) increased after both exercise protocols immediately post-exercise (HIIT: 50% and CET: 31%, respectively). HIIT increased (+22%) PC post-exercise compared to baseline and CET (p < 0.05). HIIT increased TAC immediately post-exercise (16%) and at 24h post-exercise (11%, p < 0.05), while CET increased TAC only post-exercise (12%, p < 0.05) compared to baseline, and TAC was higher following HIIT compared to CET (p < 0.05). Both HIIT and CET increased uric acid immediately post- (21% and 5%, respectively, p < 0.05) and 24h (27% and 5%, respectively, p < 0.05) post-exercise and the rise was greater following HIIT (p < 0.05). There were no significant changes (p > 0.05) for TBARS and catalase following either exercise protocol. Low-volume HIIT is associated with a greater acute phase leukocyte count and redox response than low-volume CET, and this should be considered when an exercise training program is developed and complete blood count is performed for health purposes.
单次运动即可导致炎症反应、氧化应激增加和酶抗氧化机制的上调。尽管低容量高强度间歇训练（HIIT）已经流行起来，但它对上述机制的急性反应尚未得到充分研究。本研究评估了 HIIT 对血液学特征和氧化还原状态的影响，与传统持续有氧运动（CET）相比。12 名健康年轻男性在 HIIT 和 CET 下参与随机交叉设计。在 HIIT 期间，参与者在自行车测力计上进行 4 分钟的恢复期，以 0.375 kg/kg 体重的阻力进行 4 次 30 秒冲刺。CET 由自行车测力计上 30 分钟的骑行组成，运动强度为最大摄氧量的 70%。在运动前、运动后即刻、24 小时、48 小时和 72 小时采血，并分析血液学特征和氧化还原状态（硫代巴比妥酸反应物质 [TBARS]；蛋白质羰基 [PC]；总抗氧化能力 [TAC]；过氧化氢酶和尿酸）。两种运动方案后白细胞（WBC）即刻增加（HIIT：50%，CET：31%）。与基线和 CET 相比，HIIT 运动后 PC 增加（+22%）（p<0.05）。HIIT 运动后即刻（16%）和 24 小时（11%，p<0.05）TAC 增加，而 CET 仅在运动后（12%，p<0.05）与基线相比增加，与 CET 相比，HIIT 后 TAC 更高（p<0.05）。HIIT 和 CET 后即刻（分别增加 21%和 5%，p<0.05）和 24 小时（分别增加 27%和 5%，p<0.05）尿酸增加，HIIT 后增加幅度更大（p<0.05）。两种运动方案后 TBARS 和过氧化氢酶均无显著变化（p>0.05）。低容量 HIIT 与低容量 CET 相比，急性白细胞计数和氧化还原反应更大，在制定运动训练计划和出于健康目的进行全血细胞计数时应考虑到这一点。
