Huang Rui, Zhang Jun, Ren Changjun, Zhang Xuhui, Gu Licai, Dong Yan, Zhang Juan, Zhang Jing
Department of Paediatrics.
Cell Therapy Laboratory, The First Hospital of Hebei Medical University.
Neuroreport. 2019 Mar 6;30(4):262-268. doi: 10.1097/WNR.0000000000001194.
Hypoxic-ischemic brain damage (HIBD) occurs due to intrauterine hypoxia ischemia influencing the energy supply for fetal brain cells, which affects the metabolism of the brain to make the brain suffer a severe damage. Erythropoietin (EPO), which regulates hemacytopoiesis, is a kind of cytokine. EPO is sensitive to hypoxia ischemia. In this study, we aimed to investigate the effect of EPO on the expression of Fas/FasL in brain tissues of neonatal rats with HIBD. Neonatal rats were assigned randomly to sham, HIBD, and EPO groups. Five time points for observation were 6, 12, 24, 48, and 72 h after the HIBD rat model had been established, respectively. In the HIBD group, Fas/FasL expression began to rise at 6 h, reached the peak at 12-24 h, and dropped from 24 h. In the EPO group, the expression of Fas/FasL was lower than those in HIBD group at 12, 24, and 48 h (P<0.05). Our findings suggest that EPO may reduce cell apoptosis after hypoxic-ischemic damage through reduction of the expression of Fas and FasL, and that optimal therapeutic time window is 6-24 h after HIBD.
缺氧缺血性脑损伤（HIBD）是由于子宫内缺氧缺血影响胎儿脑细胞的能量供应，进而影响大脑代谢，使大脑遭受严重损伤。促红细胞生成素（EPO）是一种调节血细胞生成的细胞因子，对缺氧缺血敏感。本研究旨在探讨EPO对HIBD新生大鼠脑组织中Fas/FasL表达的影响。将新生大鼠随机分为假手术组、HIBD组和EPO组。分别在建立HIBD大鼠模型后的6、12、24、48和72小时这五个时间点进行观察。在HIBD组中，Fas/FasL表达在6小时开始上升，在12 - 24小时达到峰值，并从24小时开始下降。在EPO组中，Fas/FasL在12、24和48小时的表达低于HIBD组（P<0.05）。我们的研究结果表明，EPO可能通过降低Fas和FasL的表达来减少缺氧缺血损伤后的细胞凋亡，且最佳治疗时间窗为HIBD后的6 - 24小时。
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