Department of Plant Pathology and Weed Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan 50250, Israel; and The Robert H. Smith Faculty of Agriculture, Food and Environment, The Levi Eshkol School of Agriculture, The Hebrew University of Jerusalem, Rehovot 761001, Israel.
Department of Plant Pathology and Weed Sciences, Agricultural Research Organization, The Volcani Center.
Plant Dis. 2018 Apr;102(4):753-759. doi: 10.1094/PDIS-07-17-1124-RE. Epub 2018 Feb 13.
In the last decade, the phenomenon of late-wilting has increased in cucumber greenhouses during Cucumber green mottle mosaic virus (CGMMV) epidemics. Because the wilting appears in defined patches accompanied by root rot, it was hypothesized that the phenomenon is caused by coinfection of soilborne pathogens and CGMMV. A field survey showed that 69% of the collapsed plants were infected with both Pythium spp. and CGMMV, whereas only 20 and 6.6% were singly infected with Pythium spp. or CGMMV, respectively. Artificial inoculations in controlled-environmental growth chambers and glasshouse experiments showed that coinfection with Pythium spinosum and CGMMV leads to a strong synergistic wilting effect and reduces growth parameters. The synergy values of the wilting effect were not influenced by the time interval between P. spinosum and CGMMV infection. However, dry mass synergy values were decreased with longer intervals between infections. The results obtained in this study support the complexity of the wilting phenomenon described in commercial cucumber grown in protected structures during infection of Pythium spp. on the background of a vast CGMMV epidemic. They encourage a wider perspective of the complexity of agricultural diseases to apply the most suitable disease management.
在过去十年中，黄瓜绿斑驳花叶病毒（CGMMV）流行期间，温室黄瓜出现了晚萎现象。由于萎蔫出现在伴有根腐病的特定斑块中，因此假设这种现象是由土传病原体和 CGMMV 的共同感染引起的。田间调查显示，69%的倒塌植物同时感染了腐霉属和 CGMMV，而单独感染腐霉属或 CGMMV 的植物分别为 20%和 6.6%。在受控环境生长室和温室实验中的人工接种表明，腐霉属和 CGMMV 的共同感染会导致强烈的协同萎蔫效应，并降低生长参数。萎蔫效应的协同值不受腐霉属和 CGMMV 感染之间时间间隔的影响。然而，随着感染间隔时间的延长，干物质协同值降低。本研究的结果支持了在广泛的 CGMMV 流行背景下，在保护结构中种植的商业黄瓜感染腐霉属时描述的萎蔫现象的复杂性。它们鼓励从更广泛的角度看待农业疾病的复杂性，以应用最合适的疾病管理。
