The University of Sydney Plant Breeding Institute, Faculty of Science, Cobbitty, NSW 2570, Australia.
Department of Economic Development, Jobs, Transport and Resources, AgriBio Centre, La Trobe Research and Development Park, Bundoora, VIC 3083, Australia.
Plant Dis. 2018 Feb;102(2):413-420. doi: 10.1094/PDIS-08-17-1144-RE. Epub 2017 Dec 14.
The Australian continent was free from wheat stripe rust caused by Puccinia striiformis f. sp. tritici until exotic incursions occurred in 1979 and 2002. The 2002 incursion enabled the identification of a new stripe rust resistance gene (Yr34) in the advanced breeding line WAWHT2046. In this study, we developed and validated markers closely linked with Yr34, which is located in the distal region in the long arm of chromosome 5A. Four kompetitive allele-specific polymerase chain reaction (KASP) and three sequence-tagged site (STS) markers derived from the International Wheat Genome Sequencing Consortium RefSeq v1.0 scaffold-77836 cosegregated with Yr34. Markers sun711, sun712, sun725, sunKASP_109, and sunKASP_112 were shown to be suitable for marker-assisted selection in a validation panel of 71 Australian spring wheat genotypes, with the exception of cultivar Orion that carried the Yr34-linked alleles for sunKASP_109 and sunKASP_112. Markers previously reported to be linked with adult plant stripe rust resistance gene Yr48 also cosegregated with Yr34. Wheat genotypes carrying Yr34 and Yr48 produced identical haplotypes for the Yr34-linked markers identified in this study and those previously reported to be linked with Yr48. Phenotypic testing of genotypes carrying Yr34 and Yr48 showed that both genes conferred similar seedling responses to pre-2002 and post-2002 P. striiformis f. sp. tritici pathotypes. Further testing of 600 F plants from a cross between WAWHT2046 and RIL143 (Yr48) with P. striiformis f. sp. tritici pathotype 134 E16A+Yr17+Yr27+ failed to reveal any susceptible segregants. Our results strongly suggest that Yr34 and Yr48 are the same gene, and that Yr48 should be considered a synonym of Yr34.
直到 1979 年和 2002 年外来入侵，澳大利亚大陆才免受由条形柄锈菌小麦专化型引起的小麦条锈病。2002 年的入侵使在先进的育种系 WAWHT2046 中鉴定出一个新的抗条锈病基因（Yr34）成为可能。在这项研究中，我们开发并验证了与 Yr34 紧密连锁的标记，该基因位于 5A 染色体长臂的远端。来自国际小麦基因组测序联盟 RefSeq v1.0 支架-77836 的四个竞争性等位基因特异性聚合酶链反应（KASP）和三个序列标记位点（STS）标记与 Yr34 共分离。标记 sun711、sun712、sun725、sunKASP_109 和 sunKASP_112 在 71 个澳大利亚春小麦基因型的验证面板中表现出适合标记辅助选择，除了携带 sunKASP_109 和 sunKASP_112 与 Yr34 连锁等位基因的 Orion 品种外。以前报道与成株期抗条锈病基因 Yr48 连锁的标记也与 Yr34 共分离。携带 Yr34 和 Yr48 的小麦基因型产生与本研究中鉴定的与 Yr34 连锁的标记以及以前报道与 Yr48 连锁的标记相同的单倍型。携带 Yr34 和 Yr48 的基因型的表型测试表明，这两个基因对 2002 年前和 2002 年后的条形柄锈菌小麦专化型表现出相似的幼苗反应。对 WAWHT2046 和 RIL143（Yr48）杂交的 600 个 F 植株与条形柄锈菌小麦专化型 134 E16A+Yr17+Yr27+进行进一步测试，未发现任何易感分离。我们的结果强烈表明，Yr34 和 Yr48 是同一个基因，Yr48 应被视为 Yr34 的同义词。
