Wu Limei, Wu Yutong, Xiong Zhengai, Yao Chenguo, Zeng Manman, Zhang Ruizhe, Hua Yuanyuan
Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, P.R. China.
State Key Laboratory of Power Transmission Equipment and System Security and New Technology, Chongqing University, Chongqing 400044, P.R. China.
Oncol Lett. 2019 Feb;17(2):1517-1522. doi: 10.3892/ol.2018.9782. Epub 2018 Nov 30.
Picosecond pulsed electric field (psPEF) is an athermal, minimally invasive and local ablative biomedical engineering technique used in cancer therapy. However, to the best of our knowledge, the effect of psPEF on angiogenesis in cervical cancer is unknown. Therefore, the aim of the current study was to investigate the effects and possible mechanism of psPEF on angiogenesis in cervical cancer . HeLa cell and human umbilical vein endothelial cell (HUVEC) suspensions were exposed to psPEF with an increasing gradient of electric field intensity (0, 200, 400 and 600 kV/cm). A Cell Counting kit-8 assay and flow cytometry were used to investigate the effect of psPEF on the proliferation and apoptosis of HUVECs. The invasion, migration and tube formation capabilities of HUVECs following psPEF treatment were investigated by Transwell invasion assay, scratch test and lumen formation assay, respectively. Changes in the protein and mRNA levels of angiogenesis-associated factors in HeLa cells were detected by western blot analysis and reverse transcription-quantitative polymerase chain reaction. psPEF was identified to inhibit proliferation and tube formation, and induce apoptosis and necrosis of HUVECs in a dose-dependent manner. psPEF was revealed to decrease the protein and mRNA expression levels of vascular endothelial growth factor and hypoxia-inducible factor 1α in HeLa cells. In summary, psPEF exhibited anti-angiogenic effects in cervical cancer by exerting direct effects on HUVECs and indirect effects on angiogenesis-associated factors in HeLa cells.
皮秒脉冲电场（psPEF）是一种用于癌症治疗的非热、微创和局部消融的生物医学工程技术。然而，据我们所知，psPEF对宫颈癌血管生成的影响尚不清楚。因此，本研究的目的是探讨psPEF对宫颈癌血管生成的影响及可能机制。将HeLa细胞和人脐静脉内皮细胞（HUVEC）悬液暴露于电场强度递增的psPEF（0、200、400和600 kV/cm）。采用细胞计数试剂盒-8法和流式细胞术研究psPEF对HUVEC增殖和凋亡的影响。分别通过Transwell侵袭试验、划痕试验和管腔形成试验研究psPEF处理后HUVECs的侵袭、迁移和管腔形成能力。通过蛋白质印迹分析和逆转录-定量聚合酶链反应检测HeLa细胞中血管生成相关因子的蛋白质和mRNA水平变化。结果表明，psPEF可抑制HUVECs增殖和管腔形成，并呈剂量依赖性诱导其凋亡和坏死。psPEF可降低HeLa细胞中血管内皮生长因子和缺氧诱导因子1α的蛋白质和mRNA表达水平。综上所述，psPEF通过对HUVECs产生直接作用以及对HeLa细胞中血管生成相关因子产生间接作用，在宫颈癌中表现出抗血管生成作用。
