Xi Ming, Wan Song, Hua Wei, Zhou Yulin, Jiang Wencong, Hu Jianxin
Department of Urology, Huadu District People's Hospital, Southern Medical University, Guangzhou, Guangdong 510530, P.R. China.
Department of Urology, Guizhou Provincial People's Hospital, Guiyang, Guizhou 550002, P.R. China.
Oncol Lett. 2019 Feb;17(2):2296-2302. doi: 10.3892/ol.2018.9828. Epub 2018 Dec 12.
Correlation of sex determining region Y-box (SOX)9 and NM23 genes with the incidence and prognosis of prostate cancer (PC) was investigated. SOX9-small interfering ribonucleic acid (siRNA) and NM23-siRNA were constructed and transfected into PC-3 cells. The expression levels of SOX9 and NM23 messenger RNAs (mRNAs) and proteins in PC-3 cells were detected via reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blotting. MTT assay was adopted to examine the proliferation ability of the transfected cells, and Transwell assay was applied to detect the migration ability of the transfected cells. Sixty-three patients with PC and 56 patients with benign prostatic hyperplasia who were treated in Huadu District People's Hospital were enrolled. Correlation analyses were conducted for the relative expression levels of SOX9 and NM23 and the Gleason grade; and the survival curves of the patient SOX9/NM23 (S/N) Cq values were plotted. The proliferation and migration abilities of PC-3 cells were remarkably reduced after low expression of SOX9 (P<0.01), while those of PC-3 cells were significantly improved after low expression of NM23 (P<0.01). Compared with that in tissues of PC patients, the relative expression of SOX9 mRNA in patients with benign prostatic hyperplasia was obviously decreased (P<0.01), while that of NM23 mRNA was significantly elevated (P<0.01). The larger the S/N was, the shorter the patient's survival time would be (P<0.05). The low expression of SOX9 gene can significantly reduce the proliferation and migration abilities of PC cells, which is negatively associated with the incidence and prognosis of patients. The low expression of NM23 gene can markedly enhance the proliferation and migration abilities of PC cells, and it is positively related to the incidence and prognosis of patients.
研究了性别决定区Y盒（SOX）9和NM23基因与前列腺癌（PC）发病率和预后的相关性。构建了SOX9小干扰核糖核酸（siRNA）和NM23-siRNA，并将其转染到PC-3细胞中。通过逆转录定量聚合酶链反应（RT-qPCR）和蛋白质印迹法检测PC-3细胞中SOX9和NM23信使核糖核酸（mRNA）及蛋白质的表达水平。采用MTT法检测转染细胞的增殖能力，采用Transwell法检测转染细胞的迁移能力。纳入了在花都区人民医院接受治疗的63例PC患者和56例良性前列腺增生患者。对SOX9和NM23的相对表达水平与Gleason分级进行相关性分析；绘制患者SOX9/NM23（S/N）Cq值的生存曲线。SOX9低表达后，PC-3细胞的增殖和迁移能力显著降低（P<0.01），而NM23低表达后，PC-3细胞的增殖和迁移能力显著提高（P<0.01）。与PC患者组织相比，良性前列腺增生患者中SOX9 mRNA的相对表达明显降低（P<0.01），而NM23 mRNA的相对表达显著升高（P<0.01）。S/N越大，患者的生存时间越短（P<0.05）。SOX9基因低表达可显著降低PC细胞的增殖和迁移能力，这与患者的发病率和预后呈负相关。NM23基因低表达可显著增强PC细胞的增殖和迁移能力，且与患者的发病率和预后呈正相关。
