[液滴数字PCR在新生儿侵袭性真菌感染快速诊断中的临床价值]
[Clinical value of droplet digital PCR in rapid diagnosis of invasive fungal infection in neonates].
作者信息
Li Hui-Tao, Lin Bing-Chun, Huang Zhi-Feng, Yang Chuan-Zhong, Huang Wei-Min
机构信息
Department of Neonatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
出版信息
Zhongguo Dang Dai Er Ke Za Zhi. 2019 Jan;21(1):45-51. doi: 10.7499/j.issn.1008-8830.2019.01.009.
OBJECTIVE
To evaluate the clinical value of droplet digital PCR (ddPCR) in rapid and accurate diagnosis of invasive fungal infection (IFI) in neonates.
METHODS
The highly conserved sequence of fungi 18S RNA was selected as the target sequence, and primers were designed to establish a ddPCR fungal detection system. Blood samples were collected from 83 neonates with high-risk factors for IFI and/or related clinical symptoms in the neonatal intensive care unit (NICU) of a hospital in Shenzhen, China. Blood culture and ddPCR were used for fungal detection.
RESULTS
The ddPCR fungal detection system had a specificity of 100% and a sensitivity of 3.2 copies/μL, and had a good reproducibility. Among the 22 blood samples from neonates with a confirmed or clinical diagnosis of IFI, 19 were detected positive by ddPCR. Among the 61 blood samples from neonates who were suspected of IFI or had no IFI, 2 were detected positive by ddPCR.
CONCLUSIONS
The ddPCR technique can be used for the detection of neonatal IFI and is a promising tool for the screening and even diagnosis of neonatal IFI.
目的
评估微滴式数字PCR（ddPCR）在新生儿侵袭性真菌感染（IFI）快速准确诊断中的临床价值。
方法
选择真菌18S RNA的高度保守序列作为靶序列，设计引物建立ddPCR真菌检测系统。收集中国深圳某医院新生儿重症监护病房（NICU）83例有IFI高危因素和/或相关临床症状的新生儿的血样。采用血培养和ddPCR进行真菌检测。
结果
ddPCR真菌检测系统特异性为100%，灵敏度为3.2拷贝/μL，重复性良好。在22例确诊或临床诊断为IFI的新生儿血样中，19例ddPCR检测呈阳性。在61例疑似IFI或无IFI的新生儿血样中，2例ddPCR检测呈阳性。
结论
ddPCR技术可用于新生儿IFI的检测，是新生儿IFI筛查乃至诊断的一种有前景的工具。
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