Wang Jing, Song Fengjuan, Ai Yongling, Hu Shanwen, Huang Zhizhou, Zhong Wenying
Key Laboratory of Biomedical Functional Materials, China Pharmaceutical University, Nanjing, China.
Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, China Pharmaceutical University, Nanjing, China.
Luminescence. 2019 Mar;34(2):205-211. doi: 10.1002/bio.3597. Epub 2019 Jan 24.
In this study, we developed a novel simple fluorescence resonance-energy transfer (FRET) system between two-color CdTe quantum dots (QDs) assisted by cetyltrimethylammonium bromide (CTAB). Mercaptopropionic (MPA)-capped CdTe QDs serving as both donors and acceptors were successfully synthesized by changing the refluxing time in aqueous solution. CTAB micelles formed in water and minimized the distance between the donors and acceptors significantly by electrostatic interactions, improving FRET efficiency. Several factors that affected the fluorescence spectra of the FRET system were investigated. The prepared FRET system was feasible as an effective fluorescent probe to detect Hg(II) in aqueous solution. At pH 7.0, a linear relationship between the quenched fluorescence intensity of orange-emitting acceptors (QDs ) and Hg(II) concentration was acquired in the range 5-250 nmol/L with a detection limit of 1.95 nmol/L. The developed method showed excellent analytical performance for Hg(II) with high sensitivity and acceptable selectivity, reproducibility and stability. This finding indicated that the method has a promising potential application for environmental monitoring. This study demonstrated the great promise of QDs for expedient, low-cost and high-sensitivity detection of Hg(II).
在本研究中，我们开发了一种新型的、简单的荧光共振能量转移（FRET）系统，该系统以十六烷基三甲基溴化铵（CTAB）为辅助，由双色碲化镉量子点（QDs）构成。通过改变水溶液中的回流时间，成功合成了既作为供体又作为受体的巯基丙酸（MPA）包覆的碲化镉量子点。CTAB胶束在水中形成，并通过静电相互作用显著缩短了供体与受体之间的距离，提高了FRET效率。研究了影响FRET系统荧光光谱的几个因素。所制备的FRET系统作为检测水溶液中Hg(II)的有效荧光探针是可行的。在pH 7.0时，橙色发射受体（量子点）的猝灭荧光强度与Hg(II)浓度在5 - 250 nmol/L范围内呈线性关系，检测限为1.95 nmol/L。所开发的方法对Hg(II)具有优异的分析性能，具有高灵敏度、可接受的选择性、重现性和稳定性。这一发现表明该方法在环境监测方面具有广阔的潜在应用前景。本研究证明了量子点在便捷、低成本和高灵敏度检测Hg(II)方面具有巨大潜力。
