College of Plant Protection, Agricultural University of Hebei, Baoding, 071001, P. R. China; and College of Tropical Agriculture and Human Resources, University of Hawaii, Honolulu, HI 96822.
Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P. R. China.
Plant Dis. 2017 Dec;101(12):1980-1989. doi: 10.1094/PDIS-04-17-0516-RE. Epub 2017 Oct 13.
Dasheen mosaic virus (DsMV) is one of the major viruses affecting taro (Colocasia esculenta) production worldwide. Whole genome sequences were determined for two DsMV strains, Hawaii Strain I (KY242358) and Hawaii Strain II (KY242359), from taro in Hawaii. They represent the first full-length coding sequences of DsMV reported from the United States. Hawaii Strains I and II were 77 and 85% identical, respectively, with other completely sequenced DsMV isolates. Hawaii Strain I was most closely related to vanilla mosaic virus (VanMV) (KX505964.1), a strain of DsMV infecting vanilla in the southern Pacific Islands. Hawaii Strain II was most closely related to a taro DsMV isolate CTCRI-II-14 (KT026108.1) from India. Phylogenetic analysis of all available DsMV isolates based on amino acid sequences of their coat protein showed some correlation between host plant and genetic diversity. Analyses of DsMV genome sequences detected three recombinants from China and India among the six isolates with known complete genome sequences. The DsMV strain NC003537.1 from China is a recombinant of KJ786965.1 from India and Hawaii Strain II. Another DsMV strain KT026108.1 is a recombinant of Hawaii Strain II and NC003537.1 from China. The third DsMV strain KJ786965.1 from India is a recombinant of Hawaii Strain II and NC003537.1 from China. To our knowledge, this is the first report of recombination events in DsMV. Both Hawaii Strains I and II of DsMV were found widespread throughout the Hawaiian islands.
芋花叶病毒（DsMV）是全球影响芋头（Colocasia esculenta）生产的主要病毒之一。从夏威夷的芋头中确定了两个 DsMV 株系，即夏威夷株系 I（KY242358）和夏威夷株系 II（KY242359）的全基因组序列。它们代表了美国首次报道的 DsMV 的全长编码序列。夏威夷株系 I 和 II 分别与其他完全测序的 DsMV 分离株具有 77%和 85%的同一性。夏威夷株系 I 与香草花叶病毒（VanMV）（KX505964.1）最为密切相关，VanMV 是一种感染南太平洋岛屿香草的 DsMV 株系。夏威夷株系 II 与来自印度的芋头 DsMV 分离株 CTCRI-II-14（KT026108.1）最为密切相关。基于外壳蛋白氨基酸序列对所有可用的 DsMV 分离株进行系统进化分析表明，宿主植物与遗传多样性之间存在一定的相关性。对六个具有已知完整基因组序列的分离株的 DsMV 基因组序列分析检测到三个来自中国和印度的重组体。中国的 DsMV 株系 NC003537.1 是印度的 KJ786965.1 和夏威夷株系 II 的重组体。另一个 DsMV 株系 KT026108.1 是夏威夷株系 II 和中国的 NC003537.1 的重组体。第三个 DsMV 株系 KJ786965.1 来自印度，是夏威夷株系 II 和中国的 NC003537.1 的重组体。据我们所知，这是 DsMV 中首次报道重组事件。DsMV 的夏威夷株系 I 和 II 均在整个夏威夷群岛广泛分布。
