Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450002, P. R. China; College of Plant Protection, China Agricultural University, Beijing 100193; and College of Resources & Environmental Science, Henan Institute of Science and Technology, Xinxiang, P. R. China.
Institute of Plant Protection, Henan Academy of Agricultural Sciences; and IPM Key Laboratory in Southern Part of North China for Ministry of Agriculture, Zhengzhou 450002, P. R. China.
Plant Dis. 2017 Dec;101(12):2098-2103. doi: 10.1094/PDIS-04-17-0524-RE. Epub 2017 Oct 9.
Sweepoviruses (a group of begomoviruses that infect plants in the family Convolvulaceae) have monopartite genomes that consist of a circular, single-stranded DNA molecule. Seventy-three complete genomic sequences of sweepoviruses were characterized from the sweet potato samples collected in China. Eight sweepovirus species, including two novel species with proposed names of Sweet potato leaf curl China virus 2 and Sweet potato leaf curl Sichuan virus 2, were identified among these samples. One species, Sweet potato leaf curl Canary virus, was first identified in China. Among the 13 identified strains of Chinese sweepoviruses, 4 were newly discovered. Sweet potato leaf curl virus had the highest frequency (53.4%) of occurrence in the sweet potato samples from China. The similarities among the 73 sweepovirus genomic sequences were between 77.6 and 100.0%. Multiple recombination events were identified, and 16 recombinant sequences were determined. Recombination was observed between different species and between different strains of the same species. Recombination breakpoints were mainly localized on the intergenic region and in three open reading frames (AC1, AV1, and AV2). This study is the first comprehensive report on the genetic diversity of sweepoviruses in China.
双生病毒（侵染旋花科植物的一类病毒）为单链 DNA 病毒，基因组为单分体基因组。本研究从中国甘薯样本中鉴定出 73 个完整的双生病毒基因组序列。在这些样本中，鉴定出了 8 种双生病毒，包括 2 种新的病毒，分别命名为甘薯曲叶中国病毒 2 株和甘薯曲叶四川病毒 2 株。其中，甘薯曲叶加利福尼亚病毒在中国是首次发现。在中国鉴定的 13 种中国双生病毒中，有 4 种是新发现的。甘薯曲叶病毒在中国甘薯样本中的出现频率最高（53.4%）。73 个双生病毒基因组序列之间的相似性在 77.6%到 100.0%之间。鉴定出了多个重组事件，确定了 16 个重组序列。观察到不同种之间以及同种不同株系之间存在重组。重组断点主要定位于基因间区和三个开放阅读框（AC1、AV1 和 AV2）。本研究是中国双生病毒遗传多样性的首次全面报告。
