Applied Chemistry Key Lab of Hebei Province, Department of Bioengineering, Yanshan University, Qinhuangdao 066004, China.
Applied Chemistry Key Lab of Hebei Province, Department of Bioengineering, Yanshan University, Qinhuangdao 066004, China; Asparagus Industry Technology Research Institute of Hebei Province, Qinhuangdao 066004, China.
J Environ Manage. 2019 Apr 1;235:70-76. doi: 10.1016/j.jenvman.2019.01.007. Epub 2019 Jan 21.
In the past decades, the microbial fuel cell (MFC) technology has caught the attention of the scientific community for its potential in transforming petroleum hydrocarbon (PHC) pollutants directly into electricity through microbial catalyzed anodic. The microbe was one of the most important factors that both influence MFCs and PHC degradation. Here we aimed to identify new microbes to expand the list of microbial species which are both electrogenic and diesel hydrocarbon degrading. In this text, we depicted a strain of microbe named E2, isolated from on the anode surface of MFC, and using diesel as sole carbon source. E2 exhibited electrochemical activity in cyclic voltammetry curve, implicating that it had electrogenic ability. E2 degraded about 50% diesel (3.26 g/L) in maximum during 8 days. Pyrosequencing of 16S rRNA gene of E2 revealed E2 was a sub-strain of Vibrio. Corresponding to salt and alkali tolerant properties of vibrio, the optimal condition for E2 in degrading diesel was 3%-4% in salinity, and pH 8-9 in mineral medium. Collectively, as a member of Gammaproteobacteria class, E2 was novel marine microbe both electricity generation and diesel degradation, which may attract its future application toward artificial microbial community construction in MFC in promoting the PHC pollution removal.
在过去几十年中，微生物燃料电池（MFC）技术因其通过微生物催化的阳极直接将石油烃（PHC）污染物转化为电能的潜力而引起了科学界的关注。微生物是影响 MFC 和 PHC 降解的最重要因素之一。在这里，我们旨在确定新的微生物，以扩大既能发电又能降解柴油烃的微生物种类列表。在本文中，我们描述了一种从 MFC 阳极表面分离出的微生物菌株 E2，它以柴油为唯一碳源。E2 在循环伏安曲线中表现出电化学活性，表明它具有发电能力。E2 在 8 天内最大降解了约 50%的柴油（3.26 g/L）。E2 的 16S rRNA 基因的焦磷酸测序显示 E2 是弧菌的一个亚株。与弧菌的耐盐碱性相对应，E2 降解柴油的最佳条件是盐度为 3%-4%，矿质培养基的 pH 值为 8-9。总的来说，作为γ变形菌纲的一员，E2 是一种新型的既能发电又能降解柴油的海洋微生物，这可能会吸引其未来在 MFC 中人工微生物群落构建方面的应用，以促进 PHC 污染的去除。
