Vilayvong Soulideth, Hando Kiyomi, Sekine Masaki, Luangxay Thitsamay, Arounlangsy Phetsamone, Xaysomphet Pheangvilay, Xayaphet Phetlammone, Thavisouk Hatthakone, Soejima Yurie, Kitagawa Masanobu, Sawabe Motoji
Department of Comprehensive Pathology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan. Email:
Asian Pac J Cancer Prev. 2019 Jan 25;20(1):243-248. doi: 10.31557/APJCP.2019.20.1.243.
Background: Pathology and laboratory medicine (PALM) services are limited in low-resource countries, such as Lao PDR. Patients with malignant pleural effusion (MPE) are not properly diagnosed and treated in these situations. The purpose of this study is to confirm the usefulness of immunocytochemistry in MPE to identify the histological type and probable primary site of cancer of MPE and to discuss its usefulness in low-resource countries, such as Laos. Methods: We retrospectively reviewed glass slides of pleural effusion sent to the Department of Pathology at the University of Health Sciences from the central hospitals for cytological screening from January 2012 to December 2016. The cytological review, cell transfer and immunocytochemical staining were performed at Tokyo Medical and Dental University. Among 81 cases of MPE from Laos, 66 cases of malignant tumors that contained enough tumor cells were included in this study, and the slides were screened with 14 primary antibodies to classify the histological type and identify the probable primary site of carcinoma. Results: Among the 66 cases, 34 cases (52%) were of female patients, and 32 cases (48%) were of male patients. The patients’ ages ranged from 28 to 88 years with an average of 58 years. The immunocytochemical study identified 32 cases (49%) of primary lung adenocarcinoma, two cases (3%) of malignant mesothelioma, one case (1.5%) of breast/gynecological carcinoma, one case (1.5%) of T cell lymphoma, and one case (1.5%) of B cell lymphoma. Twenty-nine cases (43.5%) were classified as carcinoma not otherwise specified. Pulmonary small cell carcinoma/squamous cell carcinoma and metastatic colon, prostate, and liver carcinoma were not identified among the cases. Conclusions: Immunocytochemistry is a useful ancillary method in MPE diagnostics. This method could be applied in the pathological laboratories in low- or middle-resource countries, such as Laos.
在资源匮乏的国家，如老挝人民民主共和国，病理学和检验医学（PALM）服务有限。在这些情况下，恶性胸腔积液（MPE）患者无法得到恰当的诊断和治疗。本研究的目的是证实免疫细胞化学在MPE中对于识别MPE的组织学类型和可能的癌症原发部位的有用性，并讨论其在老挝等资源匮乏国家的有用性。方法：我们回顾性分析了2012年1月至2016年12月期间从中心医院送至健康科学大学病理科进行细胞学筛查的胸腔积液玻片。细胞学检查、细胞转移和免疫细胞化学染色在东京医科齿科大学进行。在来自老挝的81例MPE病例中，本研究纳入了66例含有足够肿瘤细胞的恶性肿瘤病例，并使用14种一抗对玻片进行筛查，以分类组织学类型并识别癌的可能原发部位。结果：在这66例病例中，34例（52%）为女性患者，32例（48%）为男性患者。患者年龄范围为28至88岁，平均年龄为58岁。免疫细胞化学研究确定了32例（49%）原发性肺腺癌、2例（3%）恶性间皮瘤、1例（1.5%）乳腺/妇科癌、1例（1.5%）T细胞淋巴瘤和1例（1.5%）B细胞淋巴瘤。29例（43.5%）被分类为未另行指定的癌。病例中未发现肺小细胞癌/鳞状细胞癌以及转移性结肠癌、前列腺癌和肝癌。结论：免疫细胞化学是MPE诊断中一种有用的辅助方法。该方法可应用于老挝等中低收入国家的病理实验室。
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