Alcina A, Hargreaves A J, Avila J, Hesketh J E, Fresno M
Centro de Biologia Molecular (CSIC-UAM), Universidad Autonoma, Madrid, Spain.
Cell Biol Int Rep. 1988 Nov;12(11):979-85. doi: 10.1016/0309-1651(88)90166-x.
A rabbit serum raised against sheep erythrocyte spectrin stained mainly the flagella of epimastigote forms of the parasitic protozoan Trypanosoma cruzi. In Western blots of T. cruzi proteins solubilized with Nonidet P-40 it recognized mainly a polypeptide doublet with an apparent molecular weight of about 240 kDa, while a pre-immune rabbit serum and a tubulin monoclonal antibody did not. The results are consistent with the idea that a spectrin-like protein may be involved in cytoskeleton-membrane interactions in flagella of T. cruzi.
一种针对绵羊红细胞血影蛋白产生的兔血清，主要染色了寄生原生动物克氏锥虫前鞭毛体形式的鞭毛。在用Nonidet P - 40溶解的克氏锥虫蛋白质的Western印迹中，它主要识别出一条表观分子量约为240 kDa的多肽双峰，而免疫前兔血清和微管蛋白单克隆抗体则不能识别。这些结果与一种血影蛋白样蛋白可能参与克氏锥虫鞭毛中细胞骨架 - 膜相互作用的观点一致。
