Schneider A, Lutz H U, Marugg R, Gehr P, Seebeck T
Institut für allgemeine Mikrobiologie, Universität Bern, Switzerland.
J Cell Sci. 1988 Jun;90 ( Pt 2):307-15. doi: 10.1242/jcs.90.2.307.
A polyclonal, monospecific rabbit antibody to human erythrocyte spectrins cross-reacted with two sets of proteins (a doublet of 180/200K and a triplet of 67-66-65K; K = 10(3) Mr) in the parasitic protozoon Trypanosoma brucei brucei. Except for the 66K protein, the cross-reacting proteins are localized in the flagellum, on the basis of evidence from cell fractionation and immunofluorescence microscopy. Immunogold labelling and electron micrographs further revealed that the spectrin-like proteins are confined to the paraflagellar rod structure. The spectrin-like proteins with apparent molecular weights of 180 and 200 share homology with spectrin band 1, since V8-protease from Staphylococcus aureus generated similarly sized, antigenic peptides from these proteins. The results indicate homology between the cross-reacting proteins and human red cell spectrin.
一种针对人红细胞血影蛋白的多克隆、单特异性兔抗体，与寄生原生动物布氏布氏锥虫中的两组蛋白质发生交叉反应（一组是180/200K的双峰，另一组是67 - 66 - 65K的三峰；K = 10³ Mr）。根据细胞分级分离和免疫荧光显微镜检查的证据，除了66K蛋白质外，交叉反应蛋白定位于鞭毛中。免疫金标记和电子显微镜照片进一步显示，血影蛋白样蛋白局限于副鞭毛杆结构。表观分子量为180和200的血影蛋白样蛋白与血影蛋白带1具有同源性，因为来自金黄色葡萄球菌的V8蛋白酶从这些蛋白质中产生了大小相似的抗原性肽段。结果表明交叉反应蛋白与人红细胞血影蛋白之间存在同源性。
