Department of Pediatric Dentistry, Dental School, University of Marburg and University Medical Center Giessen and Marburg, Campus Giessen, Schlangenzahl 14, D-35392 Giessen, Germany.
Department of Operative Dentistry and Endodontics, Dental School, University of Marburg and University Medical Center Giessen and Marburg, Campus Marburg, Georg-Voigt-Str. 3, D-35039 Marburg, Germany.
Dent Mater. 2019 Mar;35(3):422-433. doi: 10.1016/j.dental.2018.12.002. Epub 2019 Jan 22.
To investigate secondary caries inhibition after dentine pre-treatment with 2% CHX, experimental addition of CHX in primer and adhesive of a 3-step adhesive system, and industrial addition od CHX in a 2-step adhesive system.
Sixty Class-V cavities were restored according to the adhesive protocol (n=12): 1) control group, Scotchbond Multi-Purpose, 3M (CTRL), 2) 2% CHX dentine pre-treatment (DENT), 3) 0.1% CHX in primer (PRIM), 4) 0.1% CHX in bonding agent (BOND), 5) Peak Universal Bond including 0.2% CHX (PEAK). Specimens were thermocycled (10,000 cycles) and inserted into a Streptococcus mutans biofilm artificial mouth (caries model). The 10-day biological loading protocol consisted of consecutive phases of demineralisation (1h) and remineralisation (5h). Evaluation under a fluorescence microscope (demineralisation) and an SEM (marginal gap) followed, at restoration margins, and at 0.3mm and 0.5mm distance from the margins, in enamel and in dentine. Total demineralization was calculated as the sum of demineralisation and substance loss due to demineralisation.
PRIM (p=0.007, mod. LSD), BOND (p=0.012, mod. LSD) and PEAK (p=0.008, mod. LSD) exhibited significantly higher total demineralisation values in enamel margins than CTRL. No significant differences were noted for total demineralisation in dentine. Regarding marginal gaps, DENT exhibited significantly lower enamel gap values compared to all other groups (p=0.001).
2% CHX as dentine pre-treatment, 0.1% or 0.2% CHX added in adhesives did not provide any antibacterial effect regarding secondary caries in dentine. On the other hand, 2% CHX dentine pre-treatment managed to limit marginal gap formation in enamel compared to the other adhesive protocols in the study.
研究 2%CHX 对牙本质预处理、3 步粘接系统中实验性添加 CHX 于底漆和粘接剂、2 步粘接系统中工业添加 CHX 对继发龋的抑制作用。
根据粘接方案（n=12）制备 60 个 V 类洞：1）对照组，Scotchbond Multi-Purpose，3M（CTRL）；2）2%CHX 牙本质预处理（DENT）；3）底漆中 0.1%CHX（PRIM）；4）粘接剂中 0.1%CHX（BOND）；5）包含 0.2%CHX 的 Peak Universal Bond（PEAK）。标本经热循环（10000 次）后插入变形链球菌生物膜人工口腔（龋病模型）。为期 10 天的生物负载方案包括连续脱矿（1h）和再矿化（5h）阶段。随后在荧光显微镜下（脱矿）和扫描电镜下（边缘间隙）进行评估，在修复边缘、距边缘 0.3mm 和 0.5mm 处，在牙釉质和牙本质上。总脱矿量计算为脱矿和脱矿引起的物质损失的总和。
PRIM（p=0.007，mod.LSD）、BOND（p=0.012，mod.LSD）和 PEAK（p=0.008，mod.LSD）牙釉质边缘的总脱矿量显著高于 CTRL。牙本质总脱矿量无显著差异。关于边缘间隙，DENT 牙釉质间隙值明显低于其他所有组（p=0.001）。
2%CHX 作为牙本质预处理，0.1%或 0.2%CHX 添加于粘接剂中，对牙本质继发龋无任何抗菌作用。另一方面，与研究中的其他粘接方案相比，2%CHX 牙本质预处理可限制牙釉质边缘间隙的形成。
