大气压化学电离气相色谱串联质谱法首次应用于血清中大麻素的测定。
First application of atmospheric-pressure chemical ionization gas chromatography tandem mass spectrometry to the determination of cannabinoids in serum.
机构信息
Unit of Forensic Medicine, Department of Diagnostics and Public Health, University of Verona, Piazzale L. A. Scuro 10, Verona, Italy.
Unit of Forensic Medicine, Department of Diagnostics and Public Health, University of Verona, Piazzale L. A. Scuro 10, Verona, Italy.
出版信息
J Chromatogr A. 2019 Apr 26;1591:147-154. doi: 10.1016/j.chroma.2019.01.041. Epub 2019 Jan 14.
The analysis of cannabinoids in blood samples is still a challenging issue for forensic laboratories, because of the low concentrations to be determined to prove that a person acted under CannabisTherefore, sensitive analytical techniques are required. This study presents the development and validation of a novel APGC-MS/MS method for the simultaneous determination of Δ9-tetrahydrocannabinol (THC), 11-hydroxy- Δ9-THC (THC-OH), 11-nor-9-carboxy- Δ9-THC (THCA), cannabidiol (CBD), cannabidiol acid (CBDA) and cannabigerol (CBG) in human serum. The developed method was fully validated according to international guidelines, with evaluation of selectivity, precision, accuracy, linearity, LODs and LOQs, extraction recovery and matrix effect. The method was linear in the range 0.2-25 ng/mL for THC, THC-OH, CBD and CBG, while for THCA and CBDA linearity was assessed in the range of 0.8-100 ng/mL and 3-100 ng/mL, respectively. The LOQs were determined in 0.2 ng/mL for THC, 0.4 ng/mL for THC-OH, 0.8 ng/mL for CBD and CBG, 1.6 ng/mL for THCA and 3 ng/mL for CBDA. The method was applied to the analysis of 15 serum samples from DUID cases. To the best of our knowledge, the present work is the first one describing an application of APGC source in the field of forensic toxicology.
对法医实验室来说，分析血液样本中的大麻素仍然是一个具有挑战性的问题，因为需要确定低浓度的大麻素以证明一个人在大麻作用下采取了行动。因此，需要使用敏感的分析技术。本研究提出了一种新的 APGC-MS/MS 方法，用于同时测定人血清中 Δ9-四氢大麻酚（THC）、11-羟基-Δ9-THC（THC-OH）、11-去甲-9-羧基-Δ9-THC（THCA）、大麻二酚（CBD）、大麻二酚酸（CBDA）和大麻萜酚（CBG）。该方法按照国际指南进行了全面验证，评估了选择性、精密度、准确度、线性、LOD 和 LOQ、提取回收率和基质效应。该方法对 THC、THC-OH、CBD 和 CBG 的线性范围为 0.2-25ng/mL，而对 THCA 和 CBDA 的线性范围分别为 0.8-100ng/mL 和 3-100ng/mL。LOQ 在 THC 为 0.2ng/mL、THC-OH 为 0.4ng/mL、CBD 和 CBG 为 0.8ng/mL、THCA 为 1.6ng/mL、CBDA 为 3ng/mL 时确定。该方法应用于 15 例 DUID 病例的血清样本分析。据我们所知，目前的工作是首次描述 APGC 源在法医毒理学领域的应用。
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