磁性氧化石墨烯纳米复合材料上固定脂肪酶 CLEAs：用于生物柴油生产的高效纳米生物催化剂。
Construction of CLEAs-lipase on magnetic graphene oxide nanocomposite: An efficient nanobiocatalyst for biodiesel production.
机构信息
Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran; Research and Technology Institute of Plant Production (RTIPP), Shahid Bahonar University of Kerman, Kerman, Iran.
Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran.
出版信息
Bioresour Technol. 2019 Apr;278:473-476. doi: 10.1016/j.biortech.2019.01.050. Epub 2019 Jan 14.
In the present work, cross linked enzyme aggregates of Lipase (CLEAs-lip) was synthesized and immobilized on the magnetic amino functionalized graphene oxide (maGO-CLEAs-lip) nanocomposites. The immobilized lipase showed a broad range of temperature activity about 40-60 °C, as compared to free enzyme. In the case of maGO-CLEAs-lip nanocomposite, the observed lower Km value state 2.25 folds affinity for the p-nitrophenyl palmitate. Enzyme activity of maGO-CLEAs-lip nanocomposite was the highest up to 5 cycles. Storage stability results displayed maGO-CLEAs-lip retained about 75% of its original activity after 30 days of incubation. Remarkably, maGO-CLEAs-lip formed the highest biodiesel construction (78%) from R. communisoil after 24 h of incubation. The biodiesel yield of this nanocomposite was 3.0 folds higher than free enzyme, making it talented as an excellent nanobiocatalyst for efficient production of biodiesel.
在本工作中，交联酶聚集体的脂肪酶（CLEAs-脂）的合成和固定在磁性氨基功能化氧化石墨烯（maGO-CLEAs-脂）纳米复合材料上。固定化脂肪酶显示出较宽的温度活性范围约 40-60°C，与游离酶相比。在 maGO-CLEAs-脂纳米复合材料的情况下，观察到较低的 Km 值状态对 p-硝基苯棕榈酸酯的亲和力增加了 2.25 倍。maGO-CLEAs-脂纳米复合材料的酶活性最高可达 5 个循环。储存稳定性结果表明，maGO-CLEAs-脂在孵育 30 天后保留了约 75%的原始活性。值得注意的是，maGO-CLEAs-脂在 24 小时的孵育后从 R. communisoil 中形成了最高的生物柴油结构（78%）。该纳米复合材料的生物柴油产率比游离酶高 3.0 倍，使其成为高效生产生物柴油的优秀纳米生物催化剂。
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