Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, 90110, Thailand.
Appl Biochem Biotechnol. 2020 Apr;190(4):1319-1332. doi: 10.1007/s12010-019-03196-7. Epub 2019 Nov 21.
The biodiesel production by enzymatic catalysis is the environmentally friendly production process. In this work, the polyacrylonitrile coated magnetic nanoparticles were prepared and used as a core supporter for producing magnetic cross-linked enzyme aggregates (mCLEAs) from Aspergillus oryzae ST11 lipase with the co-feeding of bovine serum albumin (BSA). The highest immobilized lipase activity was 0.09 U/mg-support or 81.7% of recovered activity. The resulting mCLEAs exhibited the desired characteristics; it had improved the stabilities for pH, temperature, as well as storage comparing to free enzyme. Moreover, when this biocatalyst was used for biodiesel production at the optimal conditions (30% w/w of mCLEAs, 30% w/w of water content and stepwise addition of methanol), the biodiesel conversion was 95% within 24 h of reaction, while one-step addition of methanol produced 81% conversion. The mCLEAs could be reused for 5 cycles and retained the biodiesel conversion higher than 60%.
酶催化生物柴油生产是一种环保的生产工艺。在这项工作中，制备了聚丙烯腈包覆的磁性纳米粒子，并将其用作核心载体，用于从米曲霉 ST11 脂肪酶中生产磁性交联酶聚集体（mCLEAs），同时共进料牛血清白蛋白（BSA）。固定化脂肪酶的最高活性为 0.09 U/mg-载体或回收活性的 81.7%。所得 mCLEAs 表现出所需的特性；与游离酶相比，其在 pH、温度和储存稳定性方面均得到了改善。此外，当该生物催化剂在最佳条件（mCLEAs 的 30%w/w、水含量的 30%w/w 和甲醇的分步添加）下用于生物柴油生产时，反应 24 小时内生物柴油的转化率为 95%，而一步添加甲醇的转化率为 81%。mCLEAs 可重复使用 5 次，保留的生物柴油转化率高于 60%。
