Université de Lorraine, Inserm, DCAC, F-54000, Nancy, France.
Université de Lorraine, CHRU-Nancy, Pôle "Maladies du Vieillissement, Gérontologie et Soins Palliatifs", F-54000, Nancy, France.
Sci Rep. 2019 Jan 24;9(1):685. doi: 10.1038/s41598-018-36756-8.
Mean telomere length in human leukocyte DNA samples reflects the different lengths of telomeres at the ends of the 23 chromosomes and in an admixture of cells. However, only rudimentary information is available regarding the distribution of telomere lengths in all chromosomes and the different cell types in leukocyte samples. Understanding the configuration of leukocyte telomere length distribution (LTLD) could be helpful in capturing intrinsic elements that are not provided by the mean leukocyte telomere length (mLTL). The objective of this study was to analyse LTLD and its temporal variation in adults. Leukocyte samples were donated on two occasions (8 years apart) by 72 participants in the ADELAHYDE study. Telomere length was measured by Southern blotting of the terminal restriction fragments. Individuals with comparable mLTLs displayed different shapes of LTLDs. Inter-individual variation in LTLD shape was much larger than intra-individual variation in LTLD shape between baseline and follow-up leukocyte samples. These results show an important individual stability of LTLD shape over time indicating that each individual has a characteristic LTLD signature.
人类白细胞 DNA 样本中的平均端粒长度反映了 23 条染色体末端和混合细胞中端粒的不同长度。然而，关于白细胞样本中所有染色体和不同细胞类型的端粒长度分布（LTLD）的分布信息还很有限。了解白细胞端粒长度分布（LTLD）的配置情况可能有助于捕捉平均白细胞端粒长度（mLTL）所不能提供的内在因素。本研究的目的是分析成年人的 LTLD 及其时间变化。ADELAHYDE 研究中的 72 名参与者两次（相隔 8 年）捐献了白细胞样本。通过末端限制片段的 Southern 印迹法测量端粒长度。具有可比 mLTL 的个体表现出不同形状的 LTLD。LTLD 形状的个体间变异比基线和随访白细胞样本之间 LTLD 形状的个体内变异大得多。这些结果表明 LTLD 形状在时间上具有重要的个体稳定性，表明每个个体都有其特征性的 LTLD 特征。
