Kim Kyung-Hee, Kim Woo-Jin, Park Choul-Ji, Park Jong-Won, Noh Gyeong Eon, Lee Seunghyung, Lee Young Mee, Kim Hyun Chul
Genetics and Breeding Research Center, National Institute of Fisheries Science (NIFS), Geojesi 53334, Korea.
Dev Reprod. 2018 Dec;22(4):341-350. doi: 10.12717/DR.2018.22.4.341. Epub 2018 Dec 31.
Chemokines is a small protein that plays a major role in inflammatory reactions and viral infections as a chemotactic factor of cytokines involved in innate immunity. Most of the chemokines belong to the chemokine groups CC and CXC. To investigate the immune system of the olive flounder (), an expression pattern specifically induced in the early developmental stages of analysis is examined using qRT-PCR. We also examined tissue-specific expression of both CC and CXC chemokine in healthy olive flounder samples. CC and CXC chemokine shows increased expression after immune-related organs are formed compared to expression during early development. CC chemokine was more highly expressed in the fin, but CXC chemokine showed higher expression in the gills, spleen, intestines, and stomach. Spatial and temporal expression analysis of CC and CXC chemokine were performed following viral hemorrhagic septicemia virus (VHSV) infection. CC chemokine showed high expression in the gills, which are respiratory organs, whereas CXC chemokine was more highly expressed in the kidneys, an immune-related organ. These results suggest that CC and CXC chemokine play an important role in the immune response of the olive flounder, and may be used as basic data for the immunological activity and gene analysis of it as well as other fish.
趋化因子是一种小蛋白，作为参与固有免疫的细胞因子的趋化因子，在炎症反应和病毒感染中起主要作用。大多数趋化因子属于CC和CXC趋化因子组。为了研究牙鲆的免疫系统，使用qRT-PCR检测了在分析的早期发育阶段特异性诱导的表达模式。我们还检测了健康牙鲆样本中CC和CXC趋化因子的组织特异性表达。与早期发育期间的表达相比，CC和CXC趋化因子在免疫相关器官形成后表达增加。CC趋化因子在鳍中表达更高，但CXC趋化因子在鳃、脾脏、肠道和胃中表达更高。在感染病毒性出血性败血症病毒（VHSV）后，对CC和CXC趋化因子进行了时空表达分析。CC趋化因子在作为呼吸器官的鳃中高表达，而CXC趋化因子在作为免疫相关器官的肾脏中表达更高。这些结果表明，CC和CXC趋化因子在牙鲆的免疫反应中起重要作用，并且可能用作其以及其他鱼类的免疫活性和基因分析的基础数据。
