Department of Parasitology and Genetics, Institute for Medical Science, Kosin University College of Medicine, Busan, S. Korea, 34, Amnam-dong, Seo-gu, Busan, 602-703, South Korea.
Division of Sport Science, Pusan National University, Busan, Republic of Korea.
Genes Genomics. 2020 Jul;42(7):773-777. doi: 10.1007/s13258-020-00951-7. Epub 2020 May 24.
Viral hemorrhagic septicemia (VHS) is a serious viral disease that infects the olive flounder in South Korea. The Korean aquaculture industry experienced an economic loss caused by the high infectivity and mortality.
This study aimed to evaluate the infection density of VHSV in various organs of the olive flounder including spleen, liver, kidney, stomach, esophagus, intestine, gill, muscle, heart, and brain. Olive flounders were collected from a local fish farm and injected subcutaneously with 10 PFU/fish.
Each 15 fish were sampled at 0, 3, and 7 days post challenge (dpc), respectively, to perform quantitative analysis of VHSV using SYBR-green based real-time PCR in various tissues including spleen, liver, head-kidney, body-kidney, muscle, esophagus, stomach, intestine, gill, and brain.
Organs infected with VHSV were obtained after 3 and 7 days. Each organs were examined for viral infection using real-time PCR. The data obtained from this experiment revealed copy numbers higher than 10 copies per 100 ng cDNA in the spleen (15.26 ± 3.11 copies/100 ng of cDNA), muscle (11.24 ± 2.25 copies), and gill (14.23 ± 6.26 copies), but lower in liver, head-kidney, body-kidney, esophagus, brain and stomach.
The present study, together with previous data, demonstrated that the gill, spleen, and muscle are the major target organs of VHSV in olive flounder. Therefore, central monitoring of spleen, gill and muscle should be considered and might be necessary if anti-VHSV treatment is to be successful in infected olive flounder.
病毒性出血性败血症（VHS）是一种严重的病毒性疾病，感染韩国的橄榄石斑鱼。韩国水产养殖业因高传染性和高死亡率而遭受经济损失。
本研究旨在评估VHSV 在橄榄石斑鱼各器官中的感染密度，包括脾脏、肝脏、肾脏、胃、食管、肠、鳃、肌肉、心脏和大脑。从当地养鱼场采集橄榄石斑鱼并皮下注射 10 PFU/鱼。
分别在攻毒后 0、3 和 7 天（dpc）时，对 15 条鱼进行采样，使用 SYBR-green 基于实时 PCR 在包括脾脏、肝脏、头肾、体肾、肌肉、食管、胃、肠、鳃和大脑在内的各种组织中定量分析 VHSV。
攻毒后 3 和 7 天获得感染 VHSV 的器官。使用实时 PCR 检查每个器官的病毒感染情况。从该实验获得的数据显示，在脾脏（15.26 ± 3.11 拷贝/100ng cDNA）、肌肉（11.24 ± 2.25 拷贝）和鳃（14.23 ± 6.26 拷贝）中，cDNA 的拷贝数高于 10 拷贝/100ng，而在肝脏、头肾、体肾、食管、脑和胃中则较低。
本研究与以前的数据一起表明，鳃、脾脏和肌肉是橄榄石斑鱼中 VHSV 的主要靶器官。因此，如果要成功治疗感染的橄榄石斑鱼，应考虑对脾脏、鳃和肌肉进行中央监测，并且可能有必要进行监测。
