Kim Young Sik, Yoon Jong-Man
Dept. of Marine Life Technology, College of Ocean Science and Technology, Kunsan National University, Gunsan 54150, Korea.
Dept. of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University, Gunsan 54150, Korea.
Dev Reprod. 2018 Dec;22(4):393-402. doi: 10.12717/DR.2018.22.4.393. Epub 2018 Dec 31.
Genomic DNA was isolated from the (GRV) and (GRC) from Jangheung located in the southern sea of the Korean Peninsula, respectively and we performed clustering analyses, DNA polymorphisms and the genetic differences. The seven selected primers OPC-01, OPA-04, OPA-05, OPD-07, OPD-08, OPB-10, and OPD-16 generated average bandsharing (BS) value, the genetic distance and dendrogram. The size of DNA bands varies from 90 bp to 2,400 bp. The average BS value was 0.859±0.004 within GRV and 0.916±0.006 within GRC. The average BS value between two species was 0.340±0.003, ranged from 0.250 to 0.415. The dendrogram obtained by the seven primers, indicates two genetic clusters. The genetic distance between two species ranged from 0.059 to 0.513. The individual VERMICULOPHYLLA no. 07 of GRV was genetically closely related to VERMICULOPHYLLA no. 06 of GRV (genetic distance=0.059). Especially, two entities between the individual VERMICULOPHYLLA no. 10 of GRV and CHORDA no. 22 of GRC showed the longest genetic distance (0.513) in comparison with other individuals used. Accordingly, as mentioned above, PCR analysis showed that the GRV was a little more genetically diverse than the GRC species. We convinced that this DNA analysis revealed a significant genetic distance between two species pairs (<0.01).
分别从位于朝鲜半岛南部海域的长兴岛的（GRV）和（GRC）中分离出基因组DNA，并进行了聚类分析、DNA多态性和遗传差异分析。所选的7种引物OPC - 01、OPA - 04、OPA - 05、OPD - 07、OPD - 08、OPB - 10和OPD - 16产生了平均条带共享（BS）值、遗传距离和树状图。DNA条带大小从90 bp到2400 bp不等。GRV内的平均BS值为0.859±0.004，GRC内的平均BS值为0.916±0.006。两个物种之间的平均BS值为0.340±0.003，范围为0.250至0.415。由这7种引物得到的树状图显示出两个遗传簇。两个物种之间的遗传距离范围为0.059至0.513。GRV的个体VERMICULOPHYLLA编号07与GRV的个体VERMICULOPHYLLA编号06在遗传上密切相关（遗传距离 = 0.059）。特别是，与其他使用的个体相比，GRV的个体VERMICULOPHYLLA编号10和GRC的CHORDA编号22之间的两个实体显示出最长的遗传距离（0.513）。因此，如上所述，PCR分析表明GRV在遗传上比GRC物种更具多样性。我们确信这种DNA分析揭示了两个物种对之间存在显著的遗传距离（<0.01）。
