Oh Hyun, Yoon Jong-Man
Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University, Gunsan 573-701, Republic of Korea.
Dev Reprod. 2014 Mar;18(1):43-9. doi: 10.12717/DR.2014.18.1.043.
Three species of Nortamea concinua (NC) and Haliotis discus hannai (HDH) from Tongyeong and Sulculus diversicolor supertexta (SDS) are widely distributed on the coast of the Yellow Sea, southern sea and Jeju Island in the Korean Peninsula under the innate ecosystem. There is a need to understand the genetic traits and composition of three mollusk species in order to evaluate exactly the patent genetic effect. PCR analysis was performed on DNA samples extracted from a total of 21 individuals using seven decamer oligonucleotides primers. Seven primers were shown to generate the unique shared loci to each species and shared loci by the three species which could be clearly scored. A hierarchical clustering tree was constructed using similarity matrices to generate a dendrogram, which was facilitated by the Systat version 10. 236 specific loci, with an average of 56.3 per primer, were identified in the NC species. 142 specific loci, with an average of 44.7 per primer, were identified in the HDH species. Especially, 126 numbers of shared loci by the three species, with an average of 18 per primer, were observed among the three species. Especially, the decamer primer BION-75 generated 7 unique loci to each species, which were identifying each species, in 700 bp NC species. Interestingly, the primer BION-50detected 42 shared loci by the three species, major and/or minor fragments of sizes 100 bp and 150 bp, respectively, which were identical in all samples. As regards average bandsharing value (BS) results, individuals from HDH species (0.772) exhibited higher bandsharing values than did individuals from NC species (0.655). In this study, the dendrogram obtained by the seven decamer primers indicates three genetic clusters: cluster 1 (CONCINNA 01CONCINNA 07), cluster 2 (HANNAI 08HANNAI 14), cluster 3 (SUPERTEXTA 15~SUPERTEXTA 21). Comparatively, individuals of HDH species were fairly closely related to that of SDS species, as shown in the hierarchical dendrogram of genetic distances.
在韩国半岛统营的三种美丽北鲍（NC）、皱纹盘鲍（HDH）和杂色鲍（SDS），在原生生态系统下广泛分布于黄海沿岸、南海和济州岛。为了准确评估专利遗传效应，有必要了解这三种软体动物的遗传特征和组成。使用七种十聚体寡核苷酸引物对从总共21个个体中提取的DNA样本进行了PCR分析。七种引物显示能产生每个物种独特的共享位点以及三种物种共有的位点，这些位点可以清晰地计分。使用相似性矩阵构建层次聚类树以生成树状图，这由Systat 10.2版本辅助完成。在美丽北鲍物种中鉴定出236个特异性位点，每个引物平均有56.3个。在皱纹盘鲍物种中鉴定出142个特异性位点，每个引物平均有44.7个。特别是，在这三种物种中观察到126个三种物种共有的位点，每个引物平均有18个。特别是，十聚体引物BION - 75在700 bp的美丽北鲍物种中产生了每个物种7个独特的位点，这些位点可用于识别每个物种。有趣的是，引物BION - 50检测到三种物种共有的42个位点，分别为大小100 bp和150 bp的主要和/或次要片段，在所有样本中都是相同的。关于平均条带共享值（BS）结果，皱纹盘鲍物种的个体（0.772）显示出比美丽北鲍物种的个体（0.655）更高的条带共享值。在本研究中，由七种十聚体引物获得的树状图表明有三个遗传簇：簇1（CONCINNA 01CONCINNA 07），簇2（HANNAI 08HANNAI 14），簇3（SUPERTEXTA 15~SUPERTEXTA 21）。相比之下，如遗传距离的层次树状图所示，皱纹盘鲍物种的个体与杂色鲍物种的个体关系相当密切。
