Zhang Fei-Fei, Bao Shi-Jie, Ye Sheng-Jia, Wei Bin, Gong Yao
Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology. Shanghai 200011,China. E-mail:
Shanghai Kou Qiang Yi Xue. 2019 Jun;28(3):231-236.
To investigate the effect of tooth movement at different time after repair of alveolar bone defects with tissue engineering bone constructed by rabbit bone marrow mesenchymal stem cells (BMSCs) and beta-tricalcium phosphate (β-TCP).
Alveolar bone defect (6 mm×4 mm×8 mm) was made on the right side of 40 New Zealand rabbits, which was filled with tissue engineering bone constructed by BMSCs and β-TCP as experimental sides. Tooth extraction on the other side was performed as control. The mandibular second molars in both sides were moved mesially 2, 4, 8 and 12 weeks after surgery. The specimens were taken 4 weeks after exertion. The distance of mandibular second molar moved was measured. The periodontal tissues were observed after H-E staining. TRAP staining was performed and osteoclasts were counted in the periodontal tissues on the pressure side. BMP-2 immunohistochemical staining was used to observe the average optical density of periodontal tissue on tension side. The results were analyzed with SAS 8.0 software package.
The distance of tooth movement, the amount of TRAP positive cells and the optical density of BMP-2 in the experimental side of the 2-week and 4-week groups were all significantly lower than those in the control side, while there was no significant difference between the 8-week and 12-week groups.
Eight weeks after repair of alveolar bone defect with rabbit BMSCs and β-TCP is an appropriate time for orthodontic tooth movement.
探讨用兔骨髓间充质干细胞（BMSCs）和β-磷酸三钙（β-TCP）构建的组织工程骨修复牙槽骨缺损后不同时间牙齿移动的效果。
在40只新西兰兔右侧制作牙槽骨缺损（6mm×4mm×8mm），作为实验组，用BMSCs和β-TCP构建的组织工程骨填充；另一侧拔牙作为对照组。术后2、4、8和12周将两侧下颌第二磨牙近中移动。加力4周后取材，测量下颌第二磨牙移动距离，HE染色观察牙周组织，进行抗酒石酸酸性磷酸酶（TRAP）染色并计数压力侧牙周组织中的破骨细胞，采用骨形态发生蛋白-2（BMP-2）免疫组化染色观察张力侧牙周组织的平均光密度。结果用SAS 8.0软件包进行分析。
2周和4周组实验组的牙齿移动距离、TRAP阳性细胞数量和BMP-2光密度均显著低于对照组，而8周和12周组之间无显著差异。
用兔BMSCs和β-TCP修复牙槽骨缺损8周后是正畸牙齿移动的适宜时间。
