Tissue Engineering and Biomicrofluidics Laboratory, School of Biomedical Engineering, Indian Institute of Technology (Banaras Hindu University), Varanasi, Uttar Pradesh, 221005, India.
Department of Rachana Sharir, Faculty of Ayurveda, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, 221005, India.
Appl Biochem Biotechnol. 2019 Jul;188(3):750-768. doi: 10.1007/s12010-019-02958-7. Epub 2019 Jan 25.
Psyllium husk or isabgol contains xylan backbone linked with arabinose, rhamnose, and galacturonic acid units (arabinoxylans). In this study, we demonstrate the fabrication and characterization of a macroporous three-dimensional (3D) composite scaffold by mixing psyllium husk powder (PH) and gelatin (G) in different ratios, viz.100 PH, 75/25 PH/G, and 50/50 PH/G (w/w), using an EDC-NHS coupling reaction followed by freeze-drying method. The reaction was performed in aqueous as well as in alcoholic media to determine the most appropriate solvent system for this purpose. The mechanical strength of the scaffold system was improved from 151 to 438 kPa. The fabricated scaffolds exhibited enhanced structural stability, remarkable swelling capacity, and escalated cell growth and proliferation. ATR-FTIR analysis showed the presence of amide and ester bonds indicating covalent crosslinking. SEM micrographs revealed the porous nature of the scaffolds with pores ranging from 30 to 150 μm, and further pore size distribution curve indicated that 75/25 PH/G (w/w%) EDC-NHS-alcohol scaffold exhibited the best fit to the Gaussian distribution. Swelling capacity of the 100 PH EDC-NHS-alcohol scaffolds was found to be nearly 40% from its original weight in 48 h. MTT assay using fibroblast cells revealed ~ 80% cellular proliferation by 6th day within the fabricated scaffolds in comparison to control. Graphical Abstract ᅟ.
车前子壳或洋车前子含有木聚糖主链，连接阿拉伯糖、鼠李糖和半乳糖酸单元（阿拉伯木聚糖）。在这项研究中，我们通过混合车前子壳粉（PH）和明胶（G）以不同的比例（即 100 PH、75/25 PH/G 和 50/50 PH/G（w/w）），使用 EDC-NHS 偶联反应和随后的冷冻干燥方法，制备和表征了一种具有大孔三维（3D）结构的复合支架。反应在水相和醇相介质中进行，以确定最适合该目的的溶剂系统。支架系统的机械强度从 151 kPa 提高到 438 kPa。所制备的支架表现出增强的结构稳定性、显著的溶胀能力以及增加的细胞生长和增殖。ATR-FTIR 分析表明存在酰胺和酯键，表明存在共价交联。SEM 显微照片显示支架具有多孔性质，孔径范围为 30 至 150 µm，进一步的孔径分布曲线表明，75/25 PH/G（w/w%）EDC-NHS-醇支架最符合高斯分布。在 48 小时内，100 PH EDC-NHS-醇支架的溶胀能力从其原始重量增加了近 40%。使用成纤维细胞的 MTT 测定法显示，与对照组相比，在制备的支架内，第 6 天的细胞增殖率约为 80%。
