Department of Bioresource and Environmental Sciences, Faculty of Life Sciences, Kyoto Sangyo University, Kyoto, Japan.
Center for Ecological Evolutionary Developmental Biology, Kyoto Sangyo University, Kyoto, Japan.
PLoS One. 2019 Jan 25;14(1):e0210141. doi: 10.1371/journal.pone.0210141. eCollection 2019.
Plant leaves occur in diverse shapes. Divarication patterns that develop during early growths are one of key factors that determine leaf shapes. We utilized leaves of Microsorum pteropus, a semi-aquatic fern, and closely related varieties to analyze a variation in the divarication patterns. The leaves exhibited three major types of divarication: no lobes, bifurcation, and trifurcation (i.e., monopodial branching). Our investigation of their developmental processes, using time-lapse imaging, revealed localized growths and dissections of blades near each leaf apex. Restricted cell divisions responsible for the apical growths were confirmed using a pulse-chase strategy for EdU labeling assays.
植物的叶子形状各异。在早期生长过程中形成的分歧模式是决定叶子形状的关键因素之一。我们利用半水生蕨类植物Microsorum pteropus 的叶子及其密切相关的品种来分析分歧模式的变化。这些叶子表现出三种主要的分歧类型：无裂片、分叉和三分叉（即单轴分枝）。通过使用延时成像，我们研究了它们的发育过程，揭示了叶片近叶尖处的局部生长和分裂。使用 EdU 标记测定的脉冲追踪策略证实了负责顶端生长的受限细胞分裂。
