Laboratory of Pharmacology and Pharmaceutical Care, Faculty of Pharmacy, University of Coimbra, Coimbra, Portugal.
Coimbra Institute for Clinical and Biomedical Research (iCBR), Faculty of Medicine, University of Coimbra, Coimbra, Portugal.
PLoS One. 2019 Jan 25;14(1):e0211421. doi: 10.1371/journal.pone.0211421. eCollection 2019.
In this study, we aimed at performing a histomorphometric analysis of human left internal thoracic artery (ITA) samples as well as at correlating the histomorphometric findings with the clinical profile, including risk factors and medication. Distal segments of ITA were obtained from 54 patients undergoing coronary artery bypass grafting. Histological observation was performed in paraffin-embedded transverse sections of ITA through four staining protocols: hematoxylin-eosin, van Gieson, Masson's trichrome and von Kossa. Morphometric analysis included the intimal width (IW), medial width (MW) and intima/media ratio (IMR). No overt atherosclerotic lesions were observed. Mild calcifications were observed across the vascular wall layers in almost all samples. Multivariable linear regression analysis showed associations between IW and IMR and the following clinical variables: age, gender, kidney function expressed as eGFR and myocardial infarction history. Age (odds ratio = 1.16, P = 0.004), female gender (odds ratio = 11.34, P = 0.011), eGFR (odds ratio = 1.03, P = 0.059) and myocardial infarction history (odds ratio = 4.81, P = 0.040) were identified as the main clinical predictors for intimal hyperplasia. Preatherosclerotic lesions in ITA samples from patients undergoing coronary revascularization were associated not only with classical cardiovascular risk factors such as age and gender, but also with other clinical variables, namely kidney function and myocardial infarction history.
在这项研究中，我们旨在对取自 54 例行冠状动脉旁路移植术患者的左内乳动脉（ITA）样本进行组织形态计量学分析，并将组织形态计量学发现与临床资料相关联，包括风险因素和药物治疗。ITA 的远端节段取自 54 例行冠状动脉旁路移植术的患者。通过四个染色方案（苏木精-伊红、范吉森、马松三色和冯·科萨）对 ITA 的石蜡包埋横切片进行组织学观察。形态计量学分析包括内膜宽度（IW）、中膜宽度（MW）和内膜/中膜比值（IMR）。未观察到明显的动脉粥样硬化病变。几乎所有样本的血管壁各层均观察到轻度钙化。多变量线性回归分析显示，IW 和 IMR 与以下临床变量之间存在关联：年龄、性别、肾功能（以 eGFR 表示）和心肌梗死史。年龄（比值比=1.16，P=0.004）、女性（比值比=11.34，P=0.011）、eGFR（比值比=1.03，P=0.059）和心肌梗死史（比值比=4.81，P=0.040）是内膜增生的主要临床预测因子。在接受冠状动脉血运重建的患者的 ITA 样本中，动脉粥样硬化前病变不仅与年龄、性别等经典心血管危险因素有关，还与其他临床变量（即肾功能和心肌梗死史）有关。
