Khan M A I, Ali M A, Monsur M A, Kawasaki-Tanaka A, Hayashi N, Yanagihara S, Obara M, Mia M A T, Latif M A, Fukuta Y
Bangladesh Rice Research Institute, Gazipur-1701, Bangladesh.
Tottori University, 4-101 Koyama-Minami Tottori, Tottori 680-8553, Japan.
Plant Dis. 2016 Oct;100(10):2025-2033. doi: 10.1094/PDIS-12-15-1486-RE. Epub 2016 Jul 21.
The pathogenicity of 331 blast isolates (Pyricularia oryzae Cavara) collected from different regions and ecosystems for rice cultivation in Bangladesh was evaluated by compatibility on 23 differential varieties (DV), each harboring a single blast resistance gene, and susceptible 'Lijiangxintuanheigu' (LTH). A wide variation in virulence was found among the isolates, and 267 races were classified using a new designation system. Virulence of blast isolates against DV carrying the resistance genes Pia, Pib, Pit, Pik-s, Piz-t, Pi12(t), Pi19(t), and Pi20(t), as well as avirulence against those carrying Pish, Pi9, Pita-2, and Pita, was distributed widely in Bangladesh. Cluster analysis of the compatibility data on the DV initially classified the isolates into groups I and II. The virulence spectra of the two groups differed mainly according to the reactions of the DV to Pii, Pi3, Pi5(t), Pik-m, Pi1, Pik-h, Pik, Pik-p, and Pi7(t). Group I isolates were distributed mainly in rainfed lowlands, whereas group II isolates were found mainly in irrigated lowlands; however, there were no critical differences in geographic distribution of the blast isolates. In total, 26 isolates, which could be used to identify the 23 resistance genes of the DV on the basis of their reaction patterns, were selected as a set of standard differential blast isolates. To our knowledge, this is the first clear demonstration of the diversity and differentiation of blast races in Bangladesh. This information will be used to develop a durable blast protection system in that country.
通过在23个鉴别品种（DV）上的亲和性对从孟加拉国不同水稻种植区域和生态系统收集的331个稻瘟病菌株（稻梨孢菌）的致病性进行了评估，每个鉴别品种都携带一个单一的稻瘟病抗性基因，以及感病品种‘丽江新团黑谷’（LTH）。在这些菌株中发现了毒力的广泛差异，并使用新的命名系统对267个生理小种进行了分类。孟加拉国稻瘟病菌株对携带抗性基因Pia、Pib、Pit、Pik-s、Piz-t、Pi12(t)、Pi19(t)和Pi20(t)的鉴别品种的毒力，以及对携带Pish、Pi9、Pita-2和Pita抗性基因的鉴别品种的无毒性分布广泛。对鉴别品种亲和性数据的聚类分析最初将这些菌株分为I组和II组。两组的毒力谱主要根据鉴别品种对Pii、Pi3、Pi5(t)、Pik-m、Pi1、Pik-h、Pik、Pik-p和Pi7(t)的反应而有所不同。I组菌株主要分布在雨养低地，而II组菌株主要发现于灌溉低地；然而，稻瘟病菌株的地理分布没有显著差异。总共选择了26个菌株作为一组标准鉴别稻瘟病菌株，这些菌株可根据其反应模式用于鉴定鉴别品种的23个抗性基因。据我们所知，这是孟加拉国稻瘟病生理小种多样性和分化的首次明确证明。这些信息将用于该国开发持久的稻瘟病防治系统。
