Wang Dongyuan, Zhu Feng, Wang Jichun, Ju Hongguang, Yan Yongfeng, Qi Shanyan, Ou Yuping, Tian Chengli
Plant Pathology Division, Institute of Plant Protection, Jilin Academy of Agricultural Sciences, Changchun 130033, China.
Plant Pathology Division, College of Plant Protection, Jilin Agricultural University, Changchun 130118, China.
Pathogens. 2024 Feb 28;13(3):211. doi: 10.3390/pathogens13030211.
In order to understand the pathogenicity differentiation of rice blast fungus ( Cavara), a total of 206 isolates of were collected from three Japonica rice regions in Jilin Province, northeast China. Pathogenicity test showed that the reaction pattern of 25 monogenic differential varieties (MDVs) of rice ( L.) demonstrated a wide pathogenic diversity among the isolates. Those MDVs harbor 23 resistance () genes with the susceptible variety Lijiangxintuanheigu (LTH) as control. Virulent isolates of MDVs harboring genes , , , , , , (two lines), and (two lines) had high frequencies ranging from 80 to 100%, to MDVs harboring genes , (t), , , , , (t), , , and showed intermediate frequencies ranging from 40 to 80%, and to MDVs with genes , (t), (t), (t) and (t) presented low frequencies ranging only from 0 to 40%. The U-i-k-z-ta pattern of race-named criteria categorized the 206 isolates into 175 races. Sub-unit U73 for , i7 for and (t), k177 for //, z17 for (t), and ta332 for (t) were crucial on pathogenic differences in regions. Twenty-seven standard differential blast isolates (SDBIs) were selected to characterize resistance in rice accessions. This study could help to build a durable identification system against blast in the rice area of northeast China and enhance our understanding of the differentiation and diversity of blast races in the world.
为了解稻瘟病菌（卡瓦拉）的致病性分化情况，从中国东北吉林省的三个粳稻种植区共收集了206株分离株。致病性测试表明，25个水稻单基因鉴别品种（MDVs）的反应模式显示出这些分离株之间存在广泛的致病性差异。这些MDVs含有23个抗性（R）基因，以感病品种丽江新团黑谷（LTH）作为对照。携带基因Pi-1、Pi-2、Pi-3、Pi-4、Pi-5、Pi-6、Pi-7（两个株系）和Pi-9（两个株系）的MDVs的强毒分离株对这些基因的频率较高，范围从80%到100%，对携带基因Pi-10、Pi-(t)、Pi-11、Pi-12、Pi-13、Pi-14、Pi-(t)、Pi-16、Pi-17和Pi-18的MDVs显示出中等频率，范围从40%到80%，而对具有基因Pi-19、Pi-(t)、Pi-(t)、Pi-(t)和Pi-(t)的MDVs呈现出低频率，仅从0%到40%。以U-i-k-z-ta模式命名小种的标准将206个分离株分为175个小种。用于Pi-1的亚基U73、用于Pi-2和Pi-(t)的i7、用于Pi-3//Pi-9的k177、用于Pi-10的z17和用于Pi-11的ta332对区域致病性差异至关重要。选择了27个标准稻瘟病菌鉴别分离株（SDBIs）来鉴定水稻种质资源的抗性。本研究有助于建立中国东北粳稻区持久的稻瘟病鉴定体系，并增进我们对世界稻瘟病小种分化和多样性的理解。
