De Britto Savitha, Joshi Shreya M, Jogaiah Sudisha
Laboratory of Plant Healthcare and Diagnostics, P.G. Department of Biotechnology and Microbiology, Karnatak University, Dharwad - 580003, Karnataka, India.
Division of Biological Sciences, School of Science and Technology, University of Goroka, Goroka - 441, Papua New Guinea.
Biotechnol Rep (Amst). 2021 Nov 22;32:e00690. doi: 10.1016/j.btre.2021.e00690. eCollection 2021 Dec.
Elicitors are biochemicals, and the cell wall-derived elicitors from fungi can trigger defence mechanisms in plants by increasing the phytoalexin accumulation when they encounter the pathogens. The main objective of this research was to purify and characterize a cell wall elicitor from and evaluate the seed priming effect of that elicitor for inducing systemic resistance in broccoli plants against leaf spot disease. Amongst the tested TaCWE concentrations of the seed priming (5, 10, & 25 mg ml), 10.0 mg ml showed significantly () improved early emergence, the rate of germination at 94%, and observed seedling vigour of 2601. Also, elicitor (10 mg ml) treatment alone induced 57% plant protection. On the contrary, the elicitor treated and pathogen inoculated plants induced a notable 72% protection against leaf spot disease of broccoli caused by Thus, the primed seeds with elicitor showed induced disease resistance and plant growth promotion. The prominent molecule present in the purified extracted cell wall elicitor is confirmed as trehalose. The AFM analysis indicated the trehalose length and width as 10.16 µm and 2.148 µm, respectively. FTIR chromatogram further confirmed trehalose in abundance with traces of carbon, hydrogen, nitrogen, oxygen, and LC-MS profile with a single peak eluted with a retention time of 3.78 min. The findings of this study contribute to understanding better the role of trehalose, a biogenic cell-wall elicitor that can induce systemic resistance against leaf spot disease and regulate plant growth in the broccoli plants.
激发子是生物化学物质，真菌来源的细胞壁激发子在遇到病原体时，可通过增加植物抗毒素的积累来触发植物的防御机制。本研究的主要目的是从[具体来源未提及]中纯化并鉴定一种细胞壁激发子，并评估该激发子对西兰花种子进行引发处理以诱导其对叶斑病产生系统抗性的效果。在种子引发处理所测试的TaCWE浓度（5、10和25毫克/毫升）中，10.0毫克/毫升显著（[此处原文未明确具体显著程度的描述]）提高了早期出苗率，发芽率达94%，幼苗活力为2601。此外，单独使用激发子（10毫克/毫升）处理可诱导57%的植株保护率。相反，经激发子处理并接种病原体的植株对由[具体病原体未提及]引起的西兰花叶斑病表现出显著的72%的保护率。因此，用激发子引发处理的种子表现出诱导抗病性和促进植物生长的作用。纯化提取的细胞壁激发子中存在的主要分子被确认为海藻糖。原子力显微镜分析表明，海藻糖的长度和宽度分别为10.16微米和2.148微米。傅里叶变换红外光谱色谱图进一步证实了大量海藻糖的存在，同时含有微量的碳、氢、氮、氧，液相色谱 - 质谱图显示有一个单一峰，保留时间为3.78分钟。本研究结果有助于更好地理解海藻糖的作用，海藻糖是一种生物源细胞壁激发子，可诱导对叶斑病的系统抗性并调节西兰花植株的生长。
