Sun Shiying, Wu Yi, Zeng Kai, Zhao Yue
Department of Gynecology and Obstetrics, The Second Affiliated Hospital of Anhui Medical University, Hefei, 230601, Anhui, China.
Department of Pathogenic Biology, Shenyang Medical College, Shenyang, 110034, Liaoning, China.
BMC Womens Health. 2019 Jan 25;19(1):16. doi: 10.1186/s12905-019-0718-1.
SNF5 is a key protein in regulating cell proliferation and apoptosis in various cancers. However, the physiological roles of SNF5 in Endometrial carcinoma (EC), which is one of the most frequent malignancies of the female reproduction worldwide, remains unclear. This study aims to investigate the role of SNF5 and its correlation with clinicopathologic characteristics in EC.
We performed immunohistochemistry to detect the SNF5 expression in 46 endometrial carcinomas and 20 normal endometrium (non-EC) specimens, as well as analyzed the correlations between SNF5 expression and clinicopathologic features of patients using a statistics software (GraphPad Prism V6.0). Western blotting had been used to confirm the protein level of SNF5 in endometrial tissues. In addition, we evaluated the correlations between SNF5 and p21 in EC.
The positive immunostaining rate for SNF5 in EC and non-EC specimens were 65% (30/46) and 25% (5/20) respectively, and the expression of SNF5 was dramatically increased in EC compared with the normal endometrium (P < 0.01). The overexpression of SNF5 was associated with the PR levels, but not with age, FIGO stage, grade, lymphatic metastasis, myometrial invasion or ER status. Knockdown of SNF5 inhibits the expression of p21.
Our results indicate that SNF5 plays an important role of promoting oncogenesis in EC. These findings open up the possibility for various novel and effective combination therapies targeting SNF5 in the EC.
SNF5是调节多种癌症细胞增殖和凋亡的关键蛋白。然而，SNF5在子宫内膜癌（EC）中的生理作用仍不清楚，子宫内膜癌是全球女性生殖系统中最常见的恶性肿瘤之一。本研究旨在探讨SNF5在EC中的作用及其与临床病理特征的相关性。
我们进行免疫组织化学检测46例子宫内膜癌和20例正常子宫内膜（非EC）标本中SNF5的表达，并使用统计软件（GraphPad Prism V6.0）分析SNF5表达与患者临床病理特征之间的相关性。采用蛋白质印迹法确认子宫内膜组织中SNF5的蛋白水平。此外，我们评估了EC中SNF5与p21之间的相关性。
EC和非EC标本中SNF5的免疫阳性染色率分别为65%（30/46）和25%（5/20），与正常子宫内膜相比，EC中SNF5的表达显著增加（P < 0.01）。SNF5的过表达与PR水平相关，但与年龄、国际妇产科联盟（FIGO）分期、分级、淋巴转移、肌层浸润或ER状态无关。敲低SNF5可抑制p21的表达。
我们的结果表明，SNF5在EC中发挥促进肿瘤发生的重要作用。这些发现为针对EC中SNF5的各种新型有效联合治疗开辟了可能性。
