Department of Chemistry, College of Science, Northeast Forestry University, 26 Hexing Road, Harbin 150040, PR China.
College of Chemistry, Jilin University, 2699 Qianjin Street, Changchun 130012, PR China.
Talanta. 2019 May 1;196:442-448. doi: 10.1016/j.talanta.2018.12.098. Epub 2018 Dec 30.
Fluorescent carbon quantum dots are emerging as a new class of fluorescent probes due to their unique optical properties. Herein, we presented a simple, one-pot and effective preparation strategy for copper-functionalized carbon quantum dots (Cu-CDs). The Cu-CDs demonstrated excitation-independent emissions, high fluorescent quantum yield (~ 24.4%) and excellent fluorescent stability. The fluorescence of Cu-CDs could be quenched efficiently in the presence of histidine (His) due to the coordination occurring between surface Cu and imidazole side chains of His. Thus, the Cu-CDs could be used as an efficient fluorescent probe for His detection. Under optimal conditions, the fluorescent signals of Cu-CDs decreased linearly with the concentrations of His over a range of 0.1-15 µM. The detection limit was 30 nM. The proposed method provided obvious advantages of rapid response, convenience, simplicity, high selectivity and sensitivity. Moreover, good results also have been obtained for His detection in real biological fluid samples.
荧光碳量子点由于其独特的光学性质，正在成为一类新兴的荧光探针。在此，我们提出了一种简单、一锅法、有效的铜功能化碳量子点（Cu-CDs）制备策略。Cu-CDs 表现出激发独立发射、高荧光量子产率（约 24.4%）和优异的荧光稳定性。由于表面 Cu 与 His 中咪唑侧链之间的配位作用，Cu-CDs 在存在组氨酸（His）时能够有效地猝灭荧光。因此，Cu-CDs 可以用作 His 检测的有效荧光探针。在最佳条件下，Cu-CDs 的荧光信号随 His 浓度在 0.1-15 µM 范围内呈线性降低。检测限为 30 nM。该方法具有快速响应、方便、简单、高选择性和灵敏度的明显优势。此外，在真实生物流体样品中检测 His 也取得了良好的效果。
