Wu Cong-Cong, Xiao Yao, Li Hao, Mao Liang, Deng Wei-Wei, Yu Guang-Tao, Zhang Wen-Feng, Sun Zhi-Jun
The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, 430079, China.
The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, 430079, China; Department of Oral and Maxillofacial-Head Neck Surgery, School & Hospital of Stomatology, Wuhan University, Wuhan, 430079, China.
Pathol Res Pract. 2019 Apr;215(4):772-778. doi: 10.1016/j.prp.2019.01.019. Epub 2019 Jan 14.
Expression of the family with sequence similarity 3 member C (FAM3C) is necessary for the epithelial-mesenchymal transition (EMT). However, the expression level and clinicopathological significance of FAM3C in oral squamous cell carcinoma (OSCC) has not been thoroughly elucidated to date. We performed immunohistochemical staining on human OSCC specimens with FAM3C, co-inhibitory immune checkpoints, EMT markers, and cancer stem cells (CSCs) markers to analyze the expression levels and clinicopathological features of FAM3C in OSCC. There were 210 primary OSCC specimens, 69 oral epithelial dysplasia and 42 normal oral mucosae in our human OSCC tissue microarrays cohort. We observed that FAM3C expression was upregulated in OSCC compared with normal mucosa and epithelial dysplasia (P < 0.001). Moreover, patients with higher FAM3C expression levels had a worse prognosis than those with lower expression levels (P < 0.05). Also, FAM3C expression was positively correlated with the immune checkpoints PD-L1, VISTA, and B7-H4, the EMT marker Slug and the CSC markers SOX2 and ALDH1. In conclusion, these findings suggested that overexpression of FAM3C in human OSCC may predict a poor prognosis for OSCC patients.
序列相似性家族3成员C（FAM3C）的表达是上皮-间质转化（EMT）所必需的。然而，迄今为止，FAM3C在口腔鳞状细胞癌（OSCC）中的表达水平及临床病理意义尚未得到充分阐明。我们对人OSCC标本进行了FAM3C、共抑制性免疫检查点、EMT标志物和癌症干细胞（CSC）标志物的免疫组织化学染色，以分析FAM3C在OSCC中的表达水平和临床病理特征。在我们的人OSCC组织芯片队列中，有210例原发性OSCC标本、69例口腔上皮发育异常和42例正常口腔黏膜。我们观察到，与正常黏膜和上皮发育异常相比，OSCC中FAM3C表达上调（P < 0.001）。此外，FAM3C表达水平较高的患者预后比表达水平较低的患者更差（P < 0.05）。而且，FAM3C表达与免疫检查点PD-L1、VISTA和B7-H4、EMT标志物Slug以及CSC标志物SOX2和ALDH1呈正相关。总之，这些发现表明，人OSCC中FAM3C的过表达可能预示着OSCC患者预后不良。
