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Med Oral Patol Oral Cir Bucal. 2020 Nov 1;25(6):e752-e761. doi: 10.4317/medoral.23777.
The aim of this research was to assess the expression of aldehyde dehydrogenase 1 (ALDH1) and epithelial-mesenchymal transition (EMT) markers in head and neck squamous cell carcinoma (HNSCC), and to correlate them with the clinical and histopathological parameters of a patient cohort with follow-up over an 8-year period.
For this, seventeen HNSCC and non-neoplastic adjacent epithelium (AE) samples were subjected to laser microdissection and real-time PCR to evaluate the mRNA expression of ALDH1, E-cadherin (E-CAD), N-cadherin (N-CAD), and vimentin (VIM). Also, immunohistochemistry was performed for ALDH1, E-CAD, N-CAD, and VIM in the tumor center (TC), invasion front (IF), and AE of the seventeen samples. Mann-Whitney, Kruskal-Wallis and Chi-square tests were used to correlate the mRNA and immunohistochemical expression with different variables, considering p<0.05. Kaplan-Meier curves were produced for local recurrence, regional metastasis and treatment.
A mRNA overexpression of ALDH1 in primary tumors was associated with regional metastasis and a high ALDH1 immunostaining was related to metastasis and a worse patient outcome. Additionally, a favorable outcome was associated with the transition phase and an unfavorable outcome was associated with EMT event. An overall 26.9 months was observed with longer survival associated with surgery and radiotherapy.
However, due to the intense variability inherent to the indicator proteins in the EMT process, the complete profile markers related to this biological process should be continuous investigated.
本研究旨在评估头颈部鳞状细胞癌（HNSCC）中醛脱氢酶 1（ALDH1）和上皮-间质转化（EMT）标志物的表达，并将其与经过 8 年随访的患者队列的临床和组织病理学参数相关联。
为此，对 17 例 HNSCC 和非肿瘤相邻上皮（AE）样本进行激光微切割和实时 PCR，以评估 ALDH1、E-钙黏蛋白（E-CAD）、N-钙黏蛋白（N-CAD）和波形蛋白（VIM）的 mRNA 表达。还对 17 例样本的肿瘤中心（TC）、侵袭前沿（IF）和 AE 进行了 ALDH1、E-CAD、N-CAD 和 VIM 的免疫组织化学检测。采用 Mann-Whitney、Kruskal-Wallis 和 Chi-square 检验，考虑到 p<0.05，将 mRNA 和免疫组化表达与不同变量相关联。为局部复发、区域转移和治疗生成 Kaplan-Meier 曲线。
原发肿瘤中 ALDH1 的 mRNA 过表达与区域转移相关，高 ALDH1 免疫染色与转移和患者预后不良相关。此外，向过渡阶段的转变与良好的预后相关，而 EMT 事件与不良的预后相关。观察到总体 26.9 个月的生存期，手术和放疗与更长的生存期相关。
然而，由于 EMT 过程中指示蛋白固有的强烈变异性，与该生物学过程相关的完整特征标志物应持续进行研究。
