Division of Clinical Infectious Disease & Chemotherapy, Tohoku Medical and Pharmaceutical University, 4-4-1 Komatsushima, Aoba-ku, Sendai 981-8558, Japan.
Division of Clinical Infectious Disease & Chemotherapy, Tohoku Medical and Pharmaceutical University, 4-4-1 Komatsushima, Aoba-ku, Sendai 981-8558, Japan.
J Glob Antimicrob Resist. 2019 Jun;17:316-320. doi: 10.1016/j.jgar.2019.01.015. Epub 2019 Jan 24.
The aim of this study was to determine the mutant selection window (MSW) of various disinfectants against Staphylococcus aureus and Pseudomonas aeruginosa clinical isolates to determine the tendency of these strains to acquire resistance to disinfectants.
A total of 60 S. aureus isolates [30 methicillin-resistant S. aureus (MRSA) and 30 methicillin-susceptible S. aureus (MSSA)] and 30 P. aeruginosa, including 2 multidrug-resistant P. aeruginosa (MDRP), were collected in Japan. The minimum inhibitory concentrations (MICs) and mutant prevention concentrations (MPCs) of disinfectants, including sodium hypochlorite (NaOCl), against these strains were established to determine the MSW.
The MSW, MSW and MSW for sodium hypochlorite against S. aureus and P. aeruginosa were 4×, 8× and 16× MIC, respectively. Strains surviving in the sodium hypochlorite MSW remained at a concentration of ≤0.3% (≤3072μg/mL).
This is the first evaluation of the bactericidal activity against S. aureus and P. aeruginosa strains surviving in the MSW of disinfectants. Environmental disinfection at low concentrations of sodium hypochlorite does not kill micro-organisms. Proper use of sodium hypochlorite shows a bactericidal effect against various pathogenic micro-organisms and is inexpensive, making it frequently used globally.
本研究旨在确定各种消毒剂对金黄色葡萄球菌和铜绿假单胞菌临床分离株的突变选择窗（MSW），以确定这些菌株对消毒剂产生耐药性的趋势。
在日本共收集了 60 株金黄色葡萄球菌（包括 30 株耐甲氧西林金黄色葡萄球菌（MRSA）和 30 株甲氧西林敏感金黄色葡萄球菌（MSSA））和 30 株铜绿假单胞菌，其中包括 2 株多重耐药铜绿假单胞菌（MDRP）。建立了消毒剂（包括次氯酸钠（NaOCl））对这些菌株的最低抑菌浓度（MIC）和突变预防浓度（MPC），以确定 MSW。
次氯酸钠对金黄色葡萄球菌和铜绿假单胞菌的 MSW、MSW 和 MSW 分别为 4×、8×和 16×MIC。在次氯酸钠 MSW 中存活的菌株浓度仍保持在≤0.3%（≤3072μg/mL）。
这是首次评估消毒剂 MSW 中存活的金黄色葡萄球菌和铜绿假单胞菌菌株的杀菌活性。低浓度次氯酸钠环境消毒不能杀死微生物。合理使用次氯酸钠对各种致病性微生物具有杀菌作用，且价格低廉，在全球范围内得到广泛应用。
