Department of Microbiology and Cell Biology, Tokyo Metropolitan Institute of Medical Science, 2-1-6, Kamikitazawa, Setagaya-ku, Tokyo 156-8506, Japan.
Department of Microbiology and Cell Biology, Tokyo Metropolitan Institute of Medical Science, 2-1-6, Kamikitazawa, Setagaya-ku, Tokyo 156-8506, Japan.
Virology. 2019 Mar;529:101-110. doi: 10.1016/j.virol.2019.01.015. Epub 2019 Jan 19.
Avian-origin influenza viruses like H5N1 and H7N9 often cause severe symptoms with high mortality in humans. Animal models are useful for clarification of the mechanisms of pathogenicity of these infections. In this study, to expand the potential utility of the Northern tree shrew (Tupaia belangeri) for influenza virus infection, we assessed the pathogenicity of H5N1 and H7N9 avian influenza viruses in tupaia. Infectious virus was detected continuously from nasal, oral, tracheal, and conjunctival swab samples in the animals infected with these viruses. H5N1 influenza virus infection of tupaia caused severe diffuse pneumonia with fever and weight loss. In contrast, H7N9 influenza virus infection caused focal pneumonia. The severity of pneumonia was correlated with proinflammatory cytokine transcript levels. These results indicated that tupaia can be another suitable animal model for avian influenza virus research.
禽类起源的流感病毒，如 H5N1 和 H7N9，常导致人类出现严重症状和高死亡率。动物模型对于阐明这些感染的致病机制非常有用。在这项研究中，为了扩大北树鼩（Tupaia belangeri）在流感病毒感染方面的潜在应用，我们评估了 H5N1 和 H7N9 禽流感病毒在北树鼩中的致病性。从感染这些病毒的动物的鼻腔、口腔、气管和结膜拭子样本中连续检测到传染性病毒。H5N1 流感病毒感染北树鼩引起严重弥漫性肺炎，伴有发热和体重减轻。相比之下，H7N9 流感病毒感染引起局灶性肺炎。肺炎的严重程度与促炎细胞因子转录水平相关。这些结果表明，北树鼩可以成为另一种用于禽流感病毒研究的合适动物模型。
