The Center of Tree Shrew Germplasm Resources, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases, Yunnan Innovation Team of Standardization and Application Research in Tupaia Belangeri Chinensis, Kunming, China.
Bioengineered. 2021 Dec;12(1):2836-2850. doi: 10.1080/21655979.2021.1940072.
Angiotensin I-converting enzyme 2 (ACE2), type II transmembrane serine protease 2 and 4 (TMPRSS2 and TMPRSS4) are important receptors for SARS-CoV-2 infection. In this study, the full-length tree shrew gene was cloned and sequenced, and its biological information was analyzed. The expression levels of ACE2, TMPRSS2 and TMPRSS4 in various tissues or organs of the tree shrew were detected. The results showed that the full-length gene in tree shrews was 2,786 bp, and its CDS was 2,418 bp, encoding 805 amino acids. Phylogenetic analysis based on the CDS of revealed that tree shrews were more similar to rabbits (85.93%) and humans (85.47%) but far from mice (82.81%) and rats (82.58%). In silico analysis according to the binding site of SARS-CoV-2 with the ACE2 receptor of different species predicted that tree shrews had potential SARS-CoV-2 infection possibility, which was similar to that of rabbits, cats and dogs but significantly higher than that of mice and rats. In addition, various tissues or organs of tree shrews expressed ACE2, TMPRSS2 and TMPRSS4. Among them, the kidney most highly expressed ACE2, followed by the lung and liver. The esophagus, lung, liver, intestine and kidney had relatively high expression levels of TMPRSS2 and TMPRSS4. In general, we reported for the first time the expression of ACE2, TMPRSS2 and TMPRSS4 in various tissues or organs in tree shrews. Our results revealed that tree shrews could be used as a potential animal model to study the mechanism underlying SARS-CoV-2 infection.
血管紧张素转化酶 2（ACE2）、跨膜丝氨酸蛋白酶 2 和 4（TMPRSS2 和 TMPRSS4）是 SARS-CoV-2 感染的重要受体。本研究克隆并测序了树鼩全长基因，并对其生物学信息进行了分析。检测了树鼩各种组织或器官中 ACE2、TMPRSS2 和 TMPRSS4 的表达水平。结果表明，树鼩全长基因长 2786bp，其 CDS 长 2418bp，编码 805 个氨基酸。基于 CDS 的系统进化分析表明，树鼩与兔（85.93%）和人（85.47%）更为相似，但与鼠（82.81%）和大鼠（82.58%）相差甚远。根据不同物种 SARS-CoV-2 与 ACE2 受体结合位点的计算机分析预测，树鼩具有潜在的 SARS-CoV-2 感染可能性，与兔、猫和狗相似，但明显高于鼠和大鼠。此外，树鼩的各种组织或器官均表达 ACE2、TMPRSS2 和 TMPRSS4。其中，肾脏 ACE2 表达量最高，其次是肺和肝脏。食管、肺、肝、肠和肾 TMPRSS2 和 TMPRSS4 的表达水平相对较高。总之，我们首次报道了树鼩各种组织或器官中 ACE2、TMPRSS2 和 TMPRSS4 的表达。我们的结果表明，树鼩可以作为一种潜在的动物模型，用于研究 SARS-CoV-2 感染的机制。
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