College of Chemistry & Pharmacy, Northwest A&F University, Yangling, Shaanxi, 712100, China.
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 712100, China.
Anal Chim Acta. 2019 Apr 4;1052:137-144. doi: 10.1016/j.aca.2018.11.039. Epub 2018 Nov 23.
Among organic small molecules, hydrogen peroxide (HO) and hydrazine (NHNH) often cause concern because they are widely used in biological and chemical industries. Here, we present a novel probe RH-1 for colorimetric detection of NHNH and fluorescent imaging of HO. In this probe, rhodamine was used as the main skeleton due to its favorable spectroscopic performance and stable absorption. Importantly, a benzoic acid group is present in the rhodamine skeleton, which can react with NHNH by amidation. The rhodamine skeleton can also be modified with chromogens to detect HO. Results showed that RH-1 can be used for colorimetric detection of NHNH and fluorescent monitoring of HO with high selectivity and sensitivity. The detection limits for NHNH and HO were 0.27 and 0.16 μM, respectively. Moreover, RH-1 can fluorescently image HO in living cells with low cytotoxicity.
在有机小分子中，过氧化氢 (HO) 和水合肼 (NHNH) 由于广泛应用于生物和化学工业而经常引起关注。在这里，我们提出了一种新型探针 RH-1，用于 NHNH 的比色检测和 HO 的荧光成像。在该探针中，由于其良好的光谱性能和稳定的吸收，罗丹明被用作主要骨架。重要的是，罗丹明骨架中存在苯甲酸基团，可通过酰胺化与 NHNH 反应。罗丹明骨架还可以用生色团修饰以检测 HO。结果表明，RH-1 可用于 NHNH 的比色检测和 HO 的荧光监测，具有高选择性和灵敏度。NHNH 和 HO 的检测限分别为 0.27 和 0.16 μM。此外，RH-1 具有低细胞毒性，可在活细胞中荧光成像 HO。
