Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan , Jinan 250022, China.
Anal Chem. 2017 Sep 19;89(18):9918-9925. doi: 10.1021/acs.analchem.7b02149. Epub 2017 Aug 28.
Hydrazine is carcinogenic and highly toxic so that it can lead to serious environmental contamination and serious health risks although it has been extensively used as an effective propellant and an important reactive base in industry. Thus, the development of two-emission NIR fluorescent probes for rapid detection of hydrazine with high selectivity and sensitivity is of significance and of great challenge in both biological and environmental sciences. Here, we report a two-emission colorimetric fluorescent probe for the specific detection of hydrazine based on hydrazinolysis reaction under physiological conditions. In the presence of hydrazine, the probe showed an extremely remarkable fluorescence enhancement at 627 nm compared to the decrease at 814 nm excited at different wavelength in aqueous solution. This distinct difference of two emission intensities is suitable for detection of low concentration hydrazine with a detection limit of 0.38 ppb. Addition of hydrazine resulted in a remarkable color change from blue-green to red observed by the naked eye. Kinetic study indicated a fast response of the probe toward hydrazine in minutes. Furthermore, the probe can bioimage hydrazine in living HeLa cells and mice with low cytotoxicity and excellent biocompatibility.
尽管肼作为一种有效的推进剂和工业中重要的反应性碱被广泛应用，但它具有致癌性和高毒性，可能导致严重的环境污染和严重的健康风险。因此，开发具有高选择性和灵敏度的双发射近红外荧光探针来快速检测肼在生物和环境科学领域具有重要意义和巨大挑战。在这里，我们报告了一种基于生理条件下水解反应的双发射比色荧光探针，用于特异性检测肼。在肼存在的情况下，探针在水溶液中于不同波长激发时，在 627nm 处表现出极其显著的荧光增强，而在 814nm 处则下降。这种两个发射强度的明显差异适用于检测低浓度肼，检测限为 0.38ppb。加入肼后，探针的颜色从蓝绿色肉眼观察到明显的红色变化。动力学研究表明，探针对肼的响应非常迅速，几分钟内即可完成。此外，该探针具有低细胞毒性和良好的生物相容性，可对活 HeLa 细胞和小鼠中的肼进行生物成像。
