Mig-14 可能通过降低外膜的通透性和促进生物膜的形成，促进伤寒沙门氏菌血清型 Typhi 对多黏菌素 B 的耐药性。
Mig-14 may contribute to Salmonella enterica serovar Typhi resistance to polymyxin B by decreasing the permeability of the outer-membrane and promoting the formation of biofilm.
机构信息
Department of Central lab, The Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu, 212002, China; Department of Biochemistry and Molecular Biology, Jiangsu University School of Medicine, Zhenjiang, Jiangsu, 212013, China.
Department of Biochemistry and Molecular Biology, Jiangsu University School of Medicine, Zhenjiang, Jiangsu, 212013, China.
出版信息
Int J Med Microbiol. 2019 Mar;309(2):143-150. doi: 10.1016/j.ijmm.2019.01.001. Epub 2019 Jan 16.
Mig-14 is essential for Salmonella enterica serovar Typhimurium (S. Typhimurium) resistance to antimicrobial peptides, including polymyxin B (PB). However, the molecular mechanism is as yet unknown. In this study, we demonstrated that mig-14 also played a crucial role in Salmonella enterica serovar Typhi (S. Typhi) resistance to PB. A series of genes associated with drug-resistance controlled by Mig-14 were identified in the presence of PB. Among which, ompF and ompC were up-regulated 8 and 6 folds in mig-14 mutant (Δmig-14) strains, respectively. Further, the deletion of ompF or/and ompC in Δmig-14 strains decreased their sensitivity to PB. Besides, the biofilm formation ability was reduced in Δmig-14 strains. Our results indicate that Mig-14 may contribute to PB resistance in S. Typhi by decreasing the permeability of the outer membrane and promoting biofilm formation.
Mig-14 对于沙门氏菌血清型 Typhimurium（S. Typhimurium）抵抗抗菌肽，包括多粘菌素 B（PB）是必不可少的。然而，其分子机制尚不清楚。在本研究中，我们证明 mig-14 对于沙门氏菌血清型 Typhi（S. Typhi）抵抗 PB 也起着至关重要的作用。在 PB 存在的情况下，确定了一系列与 Mig-14 控制的耐药性相关的基因。其中，ompF 和 ompC 在 mig-14 突变体（Δmig-14）菌株中分别上调了 8 倍和 6 倍。此外，在 Δmig-14 菌株中缺失 ompF 或/和 ompC 降低了它们对 PB 的敏感性。此外，Δmig-14 菌株的生物膜形成能力降低。我们的结果表明，Mig-14 可能通过降低外膜的通透性和促进生物膜形成来有助于 S. Typhi 对 PB 的耐药性。
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